Single-center comparative analysis of panel reactive antibodies of 280 cases detected by two methods

doi:10.3969/j.issn.2095-4344.2014.05.019

Abstract

Abstract:

BACKGROUND: Liquid chip techniques (Luminex) is a recently rising method for detecting anti-panel reactive antibody, which is characterized by high sensitivity, and strong specificity, less interference and high flux.

OBJECTIVE: To compare the sensitivity and detection difference of panel reactive antibody in serum of kidney disease patients detected by enzyme-linked immunosorbent assay and Luminex.

METHODS: Serum samples of 280 patients with kidney disease were selected. The enzyme-linked immunosorbent assay and Luminex methods were used to measure positive rate of panel reactive antibody. Chi-square test for fourfold table data was utilized for statistical analysis.

RESULTS AND CONCLUSION: The positive rates of panel reactive antibody were respectively 18.9% and 33.6% as detected by enzyme-linked immunosorbent assay and Luminex method. The positive rates of anti-HLA-I antibody and anti-HLA-II antibody were respectively 12.8% and 12.5%, as detected by enzyme-linked immunosorbent assay. The positive rates of anti-HLA-I antibody and anti-HLA-II antibody were respectively 25.0% and 20.7%, as detected by Luminex method. Positive detection rate was significantly higher in the Luminex group than that in the enzyme-linked immunosorbent assay group. Moreover, Luminex method could precisely detect the low-concentration antibody. Chi-square test for fourfold table data showed P < 0.01. Significant differences in the differences of panel reactive antibody of kidney disease patients were detected between the two methods. Results demonstrated that compared with enzyme-linked immunosorbent assay, Luminex method is more sensitive and accurate, and more suitable for clinical detection.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: kidney transplantation, enzyme-linked immunosorbent assay, microchip analytical procedures, HLA antigens

CLC Number:

R318

Kuang Guo-jie, Wu Hong-wen, Zhou Wen-qiang, Xiao Li. Single-center comparative analysis of panel reactive antibodies of 280 cases detected by two methods[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(5): 767-772.

Figures/Tables 2

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