Small interfering RNA inhibits the expression of surface antigens CD80/CD86 from mature dendritic cells

doi:10.3969/j.issn.2095-4344.2014.05.017

Abstract

Abstract:

BACKGROUND: The surface antigen CD80/CD86 on mature dendritic cells can activate helper T (Th) cells, reduce the differentiation of Th cells toward Th1 cells, and promote the differentiation of Th cells toward Th2 cells.

OBJECTIVE: To investigate the effect of small interfering RNA (siRNA) inhibiting the expression of surface antigens CD80/CD86 from asthmatic murine mature dendritic cells on Th1/Th2 type cytokines, interferon-γ and interleukin-4.

METHODS: Asthmatic model of mice was established; then bone marrow-derived mature dendritic cells were separated and cultured. The expression of CD11c, CD80 and CD86 on mature dendritic cells were examined by flow cytometry. The siRNA was transferred into mature dendritic cells of asthmatic mice, and the CD80/CD86 mRNA and protein expression before and after interference were determined by fluorescent quantitative PCR and flow cytometry. The mature dendritic cells in non-siRNA group, siRNA group and negative siRNA group were co-cultured with T cells. The interferon-γ and interleukin-4 productions were measured by enzyme-linked immunosorbent assay.

RESULTS AND CONCLUSION: (1) The expression of CD80/CD86 on the mature dendritic cells of asthmatic group was significantly higher than that in normal control group (all P < 0.05). (2) After siRNA was transferred into mature dendritic cells, the expression level of CD80/CD86 mRNA and protein in siRNA group was significantly lower than other groups (all P < 0.05). (3) After siRNA transfection, the level of interferon-γ from the supernatant of mature dendritic cells and T cells co-culture system was significantly increased in the siRNA group compared with other groups (all P < 0.05), while interleukin-4 production in the siRNA group was significantly decreased (all P < 0.05). These findings suggest that high expression of CD80/CD86 on mature dendritic cells of asthmatic mice is observed, specific siRNA can effectively inhibit the expression of CD80/CD86, thus increasing interferon-γ production and decreasing interleukin-4 production, which contributes to regulate the Th1/Th2 imbalance.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: dendritic cells, RNA, small interfering, antigens, surface, bone marrow, interferon-gamma, interleukin-4, asthma

CLC Number:

R318

Yan Zhi-dong, Yan Jia, Zhuansun Yong-xun, Chen Rui, Zhang Wei, Feng Su-ling, Li Jian-guo . Small interfering RNA inhibits the expression of surface antigens CD80/CD86 from mature dendritic cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(5): 754-760.

Figures/Tables 2

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