Umbilical cord blood mesenchymal stem cells transplantion for polymyositis/dermatomyositis: variation of Th cytokines

doi:10.3969/j.issn.2095-4344.2015.14.010

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (14): 2186-2191.doi: 10.3969/j.issn.2095-4344.2015.14.010

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Umbilical cord blood mesenchymal stem cells transplantion for polymyositis/dermatomyositis: variation of Th cytokines

Lai Qin¹, Yu Lian¹, Qiu Yong-rong², Chen Long-tian¹, Huang Jian-qing¹, Li Yu-min³, Zhang Li⁴, Wu Wei-hao¹, Wu Ai-yu¹, Luo Bi-hua¹, Tian Pan¹

¹Department of Hematology and Rheumatology, ²Department of Orthopedics, ³Department of Blood Transfusion, ⁴Department of Obstetrics, Longyan First Hospital, Affiliated Longyan First Hospital of Fujian Medical University, Longyan 364000, Fujian Province, China

Revised:2015-03-13 Online:2015-04-02 Published:2015-04-02
Contact: Yu Lian, Department of Hematology and Rheumatology, Longyan First Hospital, Affiliated Longyan First Hospital of Fujian Medical University, Longyan 364000, Fujian Province, China Qiu Yong-rong, Chief physician, Professor, Department of Orthopedics, Longyan First Hospital, Affiliated Longyan First Hospital of Fujian Medical University, Longyan 364000, Fujian Province, China
About author:Lai Qin, Associate chief physician, Department of Hematology and Rheumatology, Longyan First Hospital, Affiliated Longyan First Hospital of Fujian Medical University, Longyan 364000, Fujian Province, China Yu Lian, Chief physician, Professor, Master’s supervisor, Department of Hematology and Rheumatology, Longyan First Hospital, Affiliated Longyan First Hospital of Fujian Medical University, Longyan 364000, Fujian Province, China Lai Qin and Yu Lian contributed equally to this work.
Supported by:
Fujian Medical Innovation Subject, No. 2011-CXB-29; the Special Fund for Non-Affiliated Hospitals of Fujian Medical University, No. FZS13011Y; Key Technology Projects of Longyan City, No. 2012LY116

Abstract

Abstract:

BACKGROUND: In recent years, the application of stem cells to treat autoimmune diseases has become a hot spot. But, studies on umbilical cord blood mesenchymal stem cells transplantation for the treatment of polymyositis/dermatomyositis are rarely reported.

OBJECTIVE: To explore the immunologic mechanism of Th cytokines on the occurrence and development of polymyositis/dermatomyositis by observing the changes in serum interferon-γ, interleukin-4 and interleukin-17 in patients after umbilical cord blood mesenchymal stem cells transplantation.

METHODS: Eighty-one polymyositis/dermatomyositis patients were selected and divided into conventional therapy group (n=44) undergoing glucocorticoid and immunosuppressants therapy and cell transplantation group (n=37) undergoing intravenous infusion of umbilical cord blood mesenchymal stem cells at a density of (3.5-5.2 )×10⁷. Dosing regimen was same in the two groups. After follow-up of 1, 3, 6 months, the changes of creatine kinase and myodynamia were evaluated; after follow-up of 3 and 6 months, lung imaging was evaluated; in the cell transplantation group, interferon-γ, interleukin-4 and interleukin-17 levels were detected before treatment and at 3 and 6 months after treatment.

RESULTS AND CONCLUSION: At 1, 3, 6 months after treatment, the creatine kinase level was significantly decreased, and the muscle force grade was significantly increased in both groups (both P < 0.001). Compared with the conventional therapy group, the creatine kinase level was lower and the muscle force grade was higher in the cell transplantation group (both P < 0.001). Results from lung imaging test showed a remarkable improvement after cell transplantation, and it indicated that umbilical cord blood mesenchymal stem cells transplantation had good stability. At 6 months after transplantation, the level of interferon-γ was significantly increased, while the interleukin-4 level was decreased significantly (both P < 0.01); at 3, 6 months after cell transplantation, the levels of interleukin-17 were significantly decreased (P < 0.01). Levels of interleukin-4 and interleukin-17 were positively correlated with the level of creatine kinase at 6 months after cell transplantation (r=0.467, 0.488, both P < 0.05), but there was no obvious correlation between the levels of interferon-γ and creatine kinase (r=0.213, P > 0.05). These findings indicate umbilical cord blood mesenchymal stem cells transplantation combined with glucocorticoid and immunosuppressants therapy can adjust immune network effects and improve the immune tolerance in polymyositis/dermatomyositis patients, which is safe and effective.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Polymyositis, Dermatomyositis, Cord Blood Stem Cell Transplantation, Interferon-gamma, Interleukin-4, Interleukin-17

CLC Number:

R394.2

Lai Qin, Yu Lian, Qiu Yong-rong, Chen Long-tian, Huang Jian-qing, Li Yu-min, Zhang Li, Wu Wei-hao, Wu Ai-yu, Luo Bi-hua, Tian Pan. Umbilical cord blood mesenchymal stem cells transplantion for polymyositis/dermatomyositis: variation of Th cytokines[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2186-2191.

Figures/Tables 5

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Umbilical cord blood mesenchymal stem cells transplantion for polymyositis/dermatomyositis: variation of Th cytokines

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