Co-transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells reduces glial scars after spinal cord injury

doi:10.3969/j.issn.2095-4344.2013.42.014

Abstract

Abstract:

BACKGROUND: Previous studies have demonstrated that transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells can effectively promote the motor function and sensory function recovery of rhesus monkeys with spinal cord injury.
OBJECTIVE: To validate whether co-transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells exhibits better protective effects on spinal cord glial scar of rhesus monkeys with spinal cord injury than cell transplantation alone.
METHODS: Twelve rhesus monkeys were collected to prepare animal models of acute severe spinal cord injury using modified Allen’s method, and then randomly divided into three groups: experimental group, co-transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells; control group, simple transplantation of bone marrow mesenchymal stem cells-derived neuron-like cells; blank control group, PBS. After 5 months, paraffin specimens of the spinal cord were made for detection of morphological and compositional characteristics of glial scar, regeneration of nerve fibers in the scar, glial scar area, and average absorbance of glial fibrillary acidic protein.
RESULTS AND CONCLUSION: Glial scar in the injured spinal cord was composed of astrocytes and histocytes. Less spinal cord glial scar area and lower absorbance value could be observed in the experimental and control groups as compared with the blank control group (P < 0.05). In addition, in the blank control group, neurofilament negative fibers could be observed in the glial scar, and astrocytes proliferated obviously. The experimental and control groups showed less fibers passed through the scar area. The glial scar area and average absorbance in the experimental group was lower than that in the control group (P< 0.05). These findings suggest that compared with simple transplantation of bone marrow mesenchymal stem cells-derived neuron-like cells, co-transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells shows better protective effects spinal tissue structure after spinal cord injury, which may be one of mechanisms by which the number of glial scars is reduced to a greater extent.

Key words: spinal cord injuries, brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor, cell transplantation

CLC Number:

R318

Liu Xiao-gang, Deng Yu-bin, Cai Hui, Zhang Xin-peng, Ma Yu-lin, Wei Ke-xin . Co-transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells reduces glial scars after spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(42): 7427-7434.

Figures/Tables 4

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