The microstructure of antigen-extracted heterologous bone

doi:10.3969/j.issn.2095-4344.2013.42.001

Abstract

Abstract:

BACKGROUND: Different methods to remove immunogenicity have different effects on the spatial microstructure of antigen-extracted heterologous bone.
OBJECTIVE: To compare the microstructure of the antigen-extracted heterologous bone via different methods to provide experimental data for tissue engineering industrialization.
METHODS: Fresh cancellous bones extracted from adult sheep vertebrae were prepared into cylinders. Their long axis direction was the same with orientation of the trabeculae. After vibration washing and different-frequency ultrasound rinsing, the cylinder samples were randomly divided into three groups: in physical calcined group, the samples were defatted, decellularized and deproteinized sequentially using methanol/chloroform and hydrogen peroxide, then bathed in sodium pyrophosphate and directly calcined at 1 000 ℃ for 3 hours; in chemical group, the samples were defatted, decellularized and deproteinized sequentially using methanol/chloroform and hydrogen peroxide; in control group, the samples were dried naturally at room temperature. Microstructure of the samples in each group was analyzed and compared through determination of porosity, scanning electron microscopy observation, X-ray diffraction analysis, X-ray atomic spectroscopy elemental analysis microscopic spatial structure.
RESULTS AND CONCLUSION: The physical calcined and chemical groups maintained natural network pore system to different extents. The size of the large pore was 50-600 μm and that of the small one was about 2 μm. The porosity was 55% to 70%. Hydroxyapatite was the main component of the physical calcined group which was determined by X-ray diffraction, and a small amount of the β-Tricalcium phosphate was also determined. In the chemical group, the main component was only hydroxyapatite. The three-dimensional spatial structures of the deproteinized cancellous bones were not damaged greatly, and they had a natural pore network system. Antigen component of xenogeneic cancellous bone can be more thoroughly removed by physical calcination step. The scaffold material made by antigen-extracted heterologous bone may satisfy the demands for bone tissue-engineering scaffolds.

Key words: antigens, stents, tissue engineering, spine

CLC Number:

R318

Cui Jie, Li Zheng, He Hui-yu, Hu Yang. The microstructure of antigen-extracted heterologous bone[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(42): 7341-7348.

Figures/Tables 9

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