Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (37): 6573-6579.doi: 10.3969/j.issn.2095-4344.2013.37.006

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Intermittent negative pressure affects tendon-bone healing after anterior cruciate ligament reconstruction

Sun Zheng-ming1, Ling Ming1, Feng Wei-lou2, Dong Xiang-hui1, Liu Shi-zhang1, Yi Zhi1   

  1. 1Second Department of Orthopedics, People’s Hospital of Shaanxi Province, Xi’an  710068, Shaanxi Province, China
    2Department of Foot and Ankle Surgery, Xi’an Honghui Hospital, Xi’an  710054, Shaanxi Province, China
  • Received:2013-03-18 Revised:2013-03-25 Online:2013-09-10 Published:2013-09-10
  • About author:Sun Zheng-ming☆, M.D., Attending physician, Second Department of Orthopedics, People’s Hospital of Shaanxi Province, Xi’an 710061, Shaanxi Province, China szmnx3@aliyun.com
  • Supported by:

    Basic Research Planning Project of Shaanxi Natural Science, No. 2011JM4050*; Shaanxi Provincial Science and Technology Department*

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Abstract:

BACKGROUND: Intermittent negative pressure has been proven to promote the repairing of soft tissue and bone healing, but the effect of negative pressure on the tendon-bone healing after anterior cruciate ligament reconstruction has not been reported.
OBJECTIVE: To research the effect of intermittent negative pressure on tendon-bone healing after anterior cruciate ligament reconstruction and on the biomechanics of tendon grafts.
METHODS: A total of 24 New Zealand white rabbits were randomly selected to establish the models of anterior cruciate ligament reconstruction of autogenous semitendinosus. The hind leg of one side was selected randomly as the negative pressure group, and the joint of the negative pressure side was connected with the micro-negative pressure aspirator through drainage tube and maintained a low-intensity and intermittent negative pressure; the contralateral hind leg was as the control and connected with ordinary drainage tube. Drainage tubes of both sides were removed at the same time after 5 days. At 6 weeks after modeling, the joint fluid was drawn to detect the expression levels of interleukin-1β; femur-ligament-tibia complex was used for tension measurement of tendon graft, and histological observation of tendon-bone interface.
RESULTS AND CONCLUSION: One rabbit had joint infection, and finally 23 rabbits were included in the study.Tension measurement results showed that maximum load for breakage in negative group pressure was significantly greater than that in the control group (P < 0.05). Histological observation found that the number of osteoblasts in the negative pressure group was significantly more than that in the control group, and the difference was statistically significant (P< 0.01). Detection of synovial fluid showed that iterleukin-1β content in synovial fluid of the negative pressure group was lower than that in the control group, and the difference was statistically significant (P < 0.01). Intermittent negative pressure may play an active role in tendon-bone healing and modeling of tendon graft after anterior cruciate ligament reconstruction.

Key words: anterior cruciate ligament, negative-pressure wound therapy, tissue transplantation, osteoblats, interleukin-1

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