Expression of matrix metalloproteinases in the synovium of rat knee joint during long-distance running

doi:10.3969/j.issn.2095-4344.2013.33.002

Abstract

Abstract:

BACKGROUND: Many studies have shown that matrix metalloproteinases 1, 3, 9 and 13 play an important role in articular cartilage degeneration and destruction, but there is less special research on the articular synovium.
OBJECTIVE: To observe the effect of long-distance running on the expressions of matrix metalloproteinases 1, 3, 9 and 13 in the synovium.
METHODS: Fifteen male Wistar rats were divided into three groups: control group, tablet group and uphill group. Rats in the control group received ordinary captivity; rats in the tablet group ran on the horizontal treadmill (0°) at the speed of 1 km/h for 1 hour daily, and lasted for 45 days; rats in the uphill group daily ran on the horizontal treadmill (0°) at the speed of 1 km/h for 1 hour, and lasted for 15 days, and then the rats ran on the uphill treadmill (+20°) at the speed of 1 km/h for 1 hour daily and lasted for 30 days. The knee joint synovium injury models with varying degrees were established. The dual hind knee joints were obtained after modeling for paraffin-embedded. Then the overall sagittal slices were obtained for hematoxylin-eosin staining and immunohistochemical staining, and the experimental results were observed and analyzed.
RESULTS AND CONCLUSION: After long-distance running, the expression of matrix metalloproteinases 1 in synovium of the tablet group and uphill group was increased when compared with that of the control group (P < 0.05), but there was no significant difference between tablet group and uphill group (P > 0.05). There was no significant difference in matrix metalloproteinases 3 expression (P > 0.05). The expressions of matrix metalloproteinase 9 and matrix metalloproteinase 13 in synovium were in gradient increasing state (P < 0.05), which were lowest in the control group, increased in the tablet group and highest in the uphill group. The results indicate that long-distance running exercise can influence the normal physiological structure of rat knee joint synovium by changing the expression of matrix metalloproteinases.

Key words: tissue construction, cartilage tissue construction, synovitis, knee joint, synovial injury, matrix metalloproteinase, long-distance, running exercise, immunohistochemistry, National Natural Science Foundation of China

CLC Number:

R318

Shangguan Ya-xian, Wei Xiao-chun, Li Kai, Wei Jian-ping. Expression of matrix metalloproteinases in the synovium of rat knee joint during long-distance running[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.33.002.

Figures/Tables 6

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