Culture methods for primary cervical carcinoma cells

doi:10.3969/j.issn.2095-4344.2013.15.016

Abstract

Abstract:

BACKGROUND: In vitro isolation and culture of cervical epithelial carcinoma cells is the foundation for studying tumor etiology and treatment from the cellular level, which is the most basic aspect of cervical cancer tissue engineering research.
OBJECTIVE: To investigate the culture methods for primary cervical carcinoma cells, and passaged cell growth, proliferation and shape in vitro.
METHODS: We isolated and cultured primary cervical carcinoma cell by using enzyme joint digestion technique (0.25% trypsin and 2% type I collagenase), observed the shape of the tumor cell and condition of the cell proliferation using the inverted fluorescent microscope, and detected CK17 and Ki67 expression by immunofluorescence.
RESULTS AND CONCLUSION: Cervical epithelial carcinoma cells harvested using the enzyme joint digestion technique had a higher purity, and this technique was relatively simple to be repeated. The passaged cervical epithelial carcinoma cells also had a stable cell phenotype. CK17 positive expression indicated the cultured cells were cervical epithelial carcinoma cells and Ki67 positive expression showed that the cultured cells had the proliferative characteristics of malignant tumor cells.

Key words: tissue construction, cytology experiment in tissue construction, cell culture, cervix, epithelial cells, medium, trypsin, collagenase type Ⅰ, primary cells, in vitro culture, growth characteristics, tumor markers, CK17, Ki67, provincial grants-supported paper

CLC Number:

R318

Cheng Jing-xin, Ma Xiu-ping, Zhou Ping, Yuan Min, Huang Kun, Zhang Yi. Culture methods for primary cervical carcinoma cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.15.016.

Figures/Tables 5

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