Early diagnosis value of combination detection of four serum biomarkers for osteoarthritis

doi:10.3969/j.issn.2095-4344.2013.15.004

Abstract

Abstract:

BACKGROUND: Studies have shown that biomarkers in the diagnosis of early osteoarthritis have their unique advantages, but so far, it is not clear on application value of biomarkers in the diagnosis of early
osteoarthritis in human being.
OBJECTIVE: To investigate the value and significance as early diagnosis and condition assessment indicators of osteoarthritis by means of quantitative detection of the changes in C-telopeptide of collagen typeⅡ, matrix metalloproteinase protein-3, tumor necrosis factor-α, and interleukin-1.
METHODS: Thirty-five New Zealand white rabbits were divided into experimental group (n=28) and control group (n=7). Resection of the ligament and meniscus was performed in the experimental group for modeling. No treatment was done in the control group. C-telopeptide of collagen type Ⅱ, matrix metalloproteinase protein-3, tumor necrosis factor-α, and interleukin-1 levels were detected at 1, 2, 3, 4 weeks after modeling in the experimental group, and these biomarkers were also detected in the control group at the same time points. Knee MRI examination, joint sample general observation and histopathological observation were conducted.
RESULTS AND CONCLUSION: Levels of four biomarkers in the experimental group were significantly higher than those in the control group at each time point after modeling (P < 0.05). Histopatological changes and knee MRI variation did not occur at 1 week after operation; however, these changes appeared at 2 weeks, and strengthened at 3-4 weeks. In addition, the levels of four biomarkers began to increase at 1 week after operation. The results suggest that combination detection of C-telopeptide of collagen type II, matrix metalloproteinase protein-3, tumor necrosis factor-α, and interleukin-1 in serum can become a reference indicator for early diagnosis and assessment of osteoarthritis.

Key words: tissue construction, bone tissue construction, osteoarthritis, collagen type Ⅱ C-telopeptide, matrix metalloproteinase-3, tumor necrosis factor-alpha, interleukin-1, provincial grants-supported paper

CLC Number:

R318

Yu Wei-guang, Zhang Xin-chao, Zhu Xing-fei, Liu Zhi-yong. Early diagnosis value of combination detection of four serum biomarkers for osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.15.004.

Figures/Tables 4

2.3 股骨髁关节软骨组织病理学观察对照组：术后4周软骨基质呈均匀粉红染色，软骨细胞核呈深蓝色，软骨表面无裂隙，细胞无簇集现象，分4层有序排列：表层软骨细胞呈梭形，近似水平排列；中间层细胞圆形散在分布；柱状层细胞呈柱状排列，其下潮线清晰；钙化层细胞表面积大，散在分布，见图2A。实验组：术后1周：病理学无明显异常，见图2B。术后2 周：4层结构不明显，表层软骨细胞呈梭形，软骨陷窝内细胞核聚集现象，软骨基质染色尚均匀，软骨细胞增生及排列略紊乱，少量细胞簇集生长，潮线完整，见图2C。术后3周：表层略粗糙，细胞核聚集明显，细胞数量多，轻度纤维化，未见明显裂隙，细胞散在和簇集增生，部分潮线欠清晰，见图2D。术后4周：表层明显粗糙，细胞核聚集现象，可见裂隙及纤维化，细胞数量减少，细胞核缩、核溶、坏死明显，排列紊乱、簇集生长明显，软骨基质明显异常：染色深浅不一，软骨基质内空的软骨陷窝数目增多，潮线有中断，见图2E。 2.4 膝关节MRI图像分析对照组：术后第4周关节内无明显积液，软骨连续性欠完整，呈高信号，表面粗糙；部分缺损；周围滑膜无明显高信号带，滑膜欠完整，见图3A。实验组：造模1周：可见关节内无明显积液；软骨连续性欠完整，软骨高信号带不明显，表面粗糙不明显；滑膜壁基本完整；去除一侧半月板的部位见低信号，对侧半月板示高信号带；髁间窝部位呈低信号；ICRS分级：0-Ⅰ级，见图3B。造模2周：可见关节内少量积液，软骨连续性欠完整，软骨高信号带不明显，表面略粗糙；滑膜壁基本完整；半月板高信号带，有分离，去除一侧半月板部位信号异常不明显，对侧半月板高信号带；髁间窝部位呈轻度高信号；ICRS分级：Ⅰ-Ⅱ级，见图3C。造模3周：可见关节内积液明显；软骨连续性不完整，软骨高信号带较多，软骨表面不完整，有缺陷；滑膜壁不完整，部分滑膜壁呈高信号；去除一侧半月板的部位见低信号，对侧半月板示高信号带；髁间窝部位呈高信号；ICRS分级：Ⅱ级，见图3D。造模4周：可见关节内积液明显；软骨连续性中断、塌陷等，软骨高信号带较多，软骨表面有缺陷；滑膜壁有断裂，滑膜壁呈高信号；去除一侧半月板的部位见低信号，对侧半月板示高信号带；髁间窝部位呈高信号；ICRS分级：Ⅱ级及以上，见图3E。"

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