Expression of transforming growth factor beta receptor during mouse submandibular gland development

doi:10.3969/j.issn.2095-4344.2013.11.012

Abstract

Abstract:

BACKGROUND: The submandibular gland in mice is a typical study model for salivary development. Transforming growth factor beta is an essential growth factor during development and diseases. Till now the receptor of transforming growth factor beta and its function during salivary development has not been known well.
OBJECTIVE: To observe the expression of transforming growth factor beta typeⅠreceptor (Tgfbr1), transforming growth factor beta type Ⅱ receptor (Tgfbr2) and phospholated extracellular signal-regulated kinase 1/2 in mouse submandibular glands in order to uncover the mechanism of transforming growth factor beta signaling in gland development.
METHODS: Male and female C57BL/6J mice were mated to get embryos at embryonic day 14.5 (E14.5)
and newborn stage. The samples were fixed in 4% paraformaldehyde and processed into paraffin embedded serial sections. The sections were stained with hematoxylin and eosin and immunohistochemistry by the antibodies of Tgfbr1, Tgfbr2 and phospholated extracellular signal-regulated kinase 1/2 using standard procedures.
RESULTS AND CONCLUSION: (1) At newborn stage, the submandibular gland was located under the mandible in mice. The submandibular gland alveolus, gland duct including intercalary duct developed well. (2) At E14.5, Tgfbr1 and Tgfbr2 were expressed strongly in the submandibular glands epithelium, but not in the mesenchyme. (3) Expression of phospholated extracellular signal-regulated kinase 1/2, similar to Tgfbr1 and Tgfbr2, was only detected in the epithelium. Therefore, transforming growth factor beta might regulate extracellular signal-regulated kinase signaling in epithelial cells of the submandibular glands in order to control the submandibular gland development.

Key words: tissue construction, tissue construction and bioactive factor, submandibular gland, transforming growth factor, transforming growth factor receptor, developmental biology, salivary gland acinar, catheter, epithelial cells, mouse embryonic stage, the National Natural Science Foundation of China, tissue construction photographs-containing paper

CLC Number:

R318

Zhao Yi-bing, Huang Xiao-feng. Expression of transforming growth factor beta receptor during mouse submandibular gland development[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 1981-1986.

Figures/Tables 4

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