Abstract:

BACKGROUND: Combination detection has clinical values in the early diagnosis of BK virus associated nephropathy (BKVAN) after renal transplantation. But the studies on it are rare.
OBJECTIVE: To establish the combination detection method for BKVAN after renal transplantation, and to discuss the pathological features and clinical characteristics of BKVAN.
METHODS: Peripheral blood and urine samples of 84 renal transplantation recipients were selected. A variety of methods were used in this study that comprised the quantitative real-time PCR method of detecting BK viral load in both urine and serum samples, the Papanicolaou staining for urine bait cells, pathologically biopsy and immunohistochemistry approach for renal biopsy tissues. All the methods were used for the early diagnosis of BKVAN.
RSSULTS AND CONCLUSION: Two patients were diagnosed with BKVAN, accounting for 2.5% of the renal allograft participants. The renal function of the two confirmed BKVAN patients improved significantly following the reduction of immunosuppressive agents. The combined detection methods can improve the diagnosis of BKVAN with higher accuracy, feasibility and paved the way for further research about the relationship between the BKV pathogen infection and the loss of renal allografts.