Bone marrow mesenchymal stem cells repair Wistar rat spinal cord injury

doi:10.3969/j.issn.2095-4344.2012.49.016

Abstract

Abstract:

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells transplantation has achieved therapeutic effects in the treatment of spinal cord injury.
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cells transplantation on motor function of rats with spinal cord injury.
METHODS: 120 Wistar rats were randomly divided into four groups: (1) in the blank group, rats were not modeled but they were locally injected with 10 μL normal saline at the day of spinal cord injury; (2) in the model group, spinal cord injury models were established by modified Allen’s crack method without any treatment; (3) in the low dose treatment group, 1 mL bone marrow mesenchymal stem cells (1×109/L) were injected through the tail vein at the day of spinal cord injury; in the high dose treatment group, rats were locally injected with 10 μL bone marrow mesenchymal stem cells (4×109/L) at the day of spinal cord injury.
RESULTS AND CONCLUSION: At 1 day after injury, there was no healing in the model group and two treatment groups, and the scores of motion function in the three groups were lower than those in the blank group (P < 0.01); at 7 days after injury, the scores of motion function in the two treatment groups were increased, and the behavior scores of the two treatment groups were higher than those of the model group, but there was no significant difference (P > 0.05); at 15 and 30 days after injury, the behavior scores of the two treatment groups were higher than those of the model group (P < 0.01). At 7, 15 and 30 days after transplantation, there was significant recovery in behaviors in the two treatment groups compared with the model group. It indicates that bone marrow mesenchymal stem cells have therapeutic effects on the spinal function of rats with spinal cord injury.

CLC Number:

R394.2

Jia Quan-zhang, Li Dong-jun, Chen Yu-bing, Sun Jing-hai, Wang Feng-hua, Xu Shuang, Liu Li-ping, Gao De-xuan, Jiang Da-wei. Bone marrow mesenchymal stem cells repair Wistar rat spinal cord injury [J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9209-9213.

Figures/Tables 4

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