In vivo magnetic resonance imaging tracking of superparamagnetic iron oxide labeled rabbit bone marrow mesenchymal stem cells after subcutaneoustransplantation

doi:10.3969/j.issn.2095-4344.2012.49.015

Abstract

Abstract:

BACKGROUND: Magnetic resonance imaging with high field units has been successfully used in tracking the superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells. However, the validity of a low-field magnetic resonance imaging unit for detecting labeled cells has not been thoroughly investigated.
OBJECTIVE: To explore the feasibility of 0.2-T magnetic resonance imaging for in vivo tracking the distribution and migration of magnetically labeled bone marrow mesenchymal stem cells after subcutaneous transplantation.
METHODS: Bone mesenchymal stem cells were isolated from rabbit bone marrow. Before implantation, bone marrow mesenchymal stem cells were double labeled in vitro with superparamagnetic iron oxide and BrdU, then the cells were composited with chitosan and subcutaneously implanted in the thigh. Thighs subcutaneously implanted with unlabeled bone marrow mesenchymal stem cells and simply superparamagnetic iron oxides were used as control.
RESULTS AND CONCLUSION: The dense iron particles could be seen in the cytoplasm of the superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells through Prussian blue staining and electron microscopy detection. Subcutaneously transplanted superparamagnetic iron oxide labeled autologous rabbit bone marrow mesenchymal stem cells showed low signal changes and maintained at least 8 weeks during T2*-weighted gradient-echo sequence imaging, and the signal gradually entered into the tissue from the graft site. Prussian blue staining and BrdU immunohistochemistry showed that most of the transplanted cells remained in the original transplantation site. It indicated that bone marrow stromal stem cells could be effectively labeled with superparamagnetic iron oxide in vitro, and it was possible to in vivo track the subcutaneously transplanted superparamagnetic iron oxide labeled autologous rabbit bone marrow mesenchymal stem cells with 0.2-T magnetic resonance imaging.

CLC Number:

R394.2

Jin Xu-hong, Zhang Shou, Yang Liu,Wen Ya-ming, Duan Xiao-jun. In vivo magnetic resonance imaging tracking of superparamagnetic iron oxide labeled rabbit bone marrow mesenchymal stem cells after subcutaneoustransplantation[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9201-9208.

Figures/Tables 5

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