Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (49): 9168-9173.doi: 10.3969/j.issn.2095-4344.2012.49.009
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Guo Li-yuan1,2,3, Liu Wei-qiang1,2,3, He Wen-zhi1,2,3, Li Qing1,2,3, Sun Xiao-fang1,2,3
Received:
2012-02-22
Revised:
2012-04-05
Online:
2012-12-02
Published:
2013-01-16
Contact:
Sun Xiao-fang, Professor, Doctoral supervisor, Institute of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China; Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou 510150, Guangdong Province, China; Guangzhou Key Laboratory of Reproduction and Genetics, Guangzhou 510150, Guangdong Province, China xiaofangsun@hotmail.com
About author:
Guo Li-yuan★, Studying for master’s degree, Institute of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China; Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou 510150, Guangdong Province, China; Guangzhou Key Laboratory of Reproduction and Genetics, Guangzhou 510150, Guangdong Province, China Liyuan-guo@hotmail.com
Supported by:
the National Natural Science Foundation of China, No. 30871378*, U1132005*; Medical Scientific Research Foundation of Guangdong Province, No. A2011280*
CLC Number:
Guo Li-yuan, Liu Wei-qiang, He Wen-zhi, Li Qing, Sun Xiao-fang. Effects of different oxygen tensions on gene expression profiling of human embryonic stem cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9168-9173.
2.2 不同氧体积分数组FY-hES-7细胞的基因表达谱差异 实验利用Roche-NimbleGen Human Gene Expression Microarrays对不同氧体积分数组FY-hES-7细胞之间基因的表达情况进行对比分析,共发现低氧组比常氧组表达上调2倍以上基因1 840个,其中上调5倍以上者11个,见表1,而表达下调2倍以上基因1 676个,其中下调5倍以上者40个,见表2。 2.5 基因芯片结果的real-time PCR验证 验证结果表明基因C4orf17、LOC644286、FBF1、SLC16A3在低氧组中表达明显上调,而基因CDH11、DKK1、EN2、H19、IGFBP5、KRT17、NR2F2、ZBTB16表达明显下调,与芯片结果相似,见表1,2,由此可见实验获得的芯片结果比较可靠。"
2.4 KEGG通路分析结果 通过对不同氧体积分数组差异表达(>2倍)基因的通路分析,发现对比常氧组,低氧组差异表达基因相关的细胞通路共有14条(P < 0.05),见表5,6。 表5,6可见低氧组中表达上调的基因与细胞因子受体的相互作用(cytokine-cytokine receptor interaction),免疫反应(intestinal immune network for IgA production;antigen processing and presentation)以及造血系统(hematopoietic cell lineage)等通路的改变相关,而表达下调基因则与胚胎发育(Wnt signaling pathway),肿瘤发生(pathways in cancer;Melanoma;Melanogenesis;TGF-beta signaling pathway;p53 signaling pathway)等通路改变相关。"
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