Effects of different oxygen tensions on gene expression profiling of human embryonic stem cells

doi:10.3969/j.issn.2095-4344.2012.49.009

Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (49): 9168-9173.doi: 10.3969/j.issn.2095-4344.2012.49.009

Previous Articles Next Articles

Effects of different oxygen tensions on gene expression profiling of human embryonic stem cells

Guo Li-yuan^1,2,3, Liu Wei-qiang^1,2,3, He Wen-zhi^1,2,3, Li Qing^1,2,3, Sun Xiao-fang^1,2,3

¹Institute of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China; ²Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou 510150, Guangdong Province, China; ³Guangzhou Key Laboratory of Reproduction and Genetics, Guangzhou 510150, Guangdong Province, China

Received:2012-02-22 Revised:2012-04-05 Online:2012-12-02 Published:2012-04-05
Contact: Sun Xiao-fang, Professor, Doctoral supervisor, Institute of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China; Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou 510150, Guangdong Province, China; Guangzhou Key Laboratory of Reproduction and Genetics, Guangzhou 510150, Guangdong Province, China xiaofangsun@hotmail.com
About author:Guo Li-yuan★, Studying for master’s degree, Institute of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China; Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou 510150, Guangdong Province, China; Guangzhou Key Laboratory of Reproduction and Genetics, Guangzhou 510150, Guangdong Province, China Liyuan-guo@hotmail.com
Supported by:
the National Natural Science Foundation of China, No. 30871378*, U1132005*; Medical Scientific Research Foundation of Guangdong Province, No. A2011280*

Abstract

Abstract:

BACKGROUND: The studies describing culture of human embryonic stem cells under hypoxic condition mainly focus on the maintenance of pluripotency and differenciation. There are few studies regarding the effects of hypoxia on gene expression in human embryonic stem cells.
OBJECTIVE: To investigate the effects of different oxygen tensions on gene expression profiling of human embryonic stem cells.
METHODS: Gene expression profiling determined by Human Gene Expression Microarrays and differentially expressed genes were analyzed by gene ontology and pathway analysis in one human embryonic stem cell line (FY-hES-7) following prolonged passage under hypoxia (5%O2) and normoxia (21%O2), respectively.
RESULTS AND COCLUSION: 1 840 upregulated genes (more than two folds) and 1676 downregulated genes (more than two folds) were identified in the hypoxic group as compared to the nomorxic group. Gene ontology analysis and pathway analysis showed that gene upregulation in the hypoxic group was associated with cell surface receptor linked signal transduction, immune response, ion transport, metabolic process, cell activation and some pathways such as cytokine-cytokine interaction, immune response, but gene downregulation in the hypoxic group was related to transcription regulation, transcription DNA-dependent regulation, neuronal differentiation, cell morphogenesis, embryonic morphogenesis, embryonic organ and system development and the pathways of embryo development and cancer. The gene expression profiling of human embryonic stem cells in different O2 tensions is variable and the functional analyses of differentially expressed genes in hypoxia are beneficial for human embryonic stem cell culture by keeping stable self-renewal capacity, preventing human embryonic stem cell differentiation and reducing the risk of tumor formation.

CLC Number:

R394.2

Guo Li-yuan, Liu Wei-qiang, He Wen-zhi, Li Qing, Sun Xiao-fang. Effects of different oxygen tensions on gene expression profiling of human embryonic stem cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9168-9173.

Figures/Tables 6

References

[1] Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282(5391):1145-1147.

[2] Westfall SD, Sachdev S, Das P, et al. Identification of oxygen-sensitive transcriptional programs in human embryonic stem cells. Stem Cells Dev. 2008;17(5):869-881.

[3] Forristal CE, Wright KL, Hanley NA, et al. Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions. Reproduction. 2010;139(1):85-97.

[4] Cowan CA, Klimanskaya I, McMahon J, et al. Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med. 2004;350(13):1353-1356.

[5] Forsyth NR, Musio A, Vezzoni P, et al. Physiologic oxygen enhances human embryonic stem cell clonal recovery and reduces chromosomal abnormalities. Cloning Stem Cells. 2006;8(1):16-23.

[6] Lim HJ, Han J, Woo DH, et al. Biochemical and morphological effects of hypoxic environment on human embryonic stem cells in long-term culture and differentiating embryoid bodies. Mol Cells. 2011;31(2):123-132.

[7] Millman JR, Tan JH, Colton CK. The effects of low oxygen on self-renewal and differentiation of embryonic stem cells. Curr Opin Organ Transplant. 2009;14(6):694-700.

[8] Zachar V, Prasad SM, Weli SC, et al. The effect of human embryonic stem cells (hESCs) long-term normoxic and hypoxic cultures on the maintenance of pluripotency. In Vitro Cell Dev Biol Anim. 2010;46(3-4):276-283.

[9] Ezashi T, Das P, Roberts RM. Roberts, Low O2 tensions and the prevention of differentiation of hES cells. Proc Natl Acad Sci U S A. 2005;102(13):4783-4788.

[10] Prasad SM, Czepiel M, Cetinkaya C, et al. Continuous hypoxic culturing maintains activation of Notch and allows long-term propagation of human embryonic stem cells without spontaneous differentiation. Cell Prolif. 2009;42(1):63-74.

[11] Forsyth NR, Kay A, Hampson K, et al. Transcriptome alterations due to physiological normoxic (2% O2) culture of human embryonic stem cells. Regen Med. 2008;3(6): 817-833.

[12] Simon MC, Keith B. The role of oxygen availability in embryonic development and stem cell function. Nat Rev Mol Cell Biol. 2008;9(4):285-296.

[13] Sun X, Long X, Yin Y, et al. Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes. Hum Reprod. 2008;23(10):2185-2193.

[14] Chen HF, Kuo HC, Chen W, et al. A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals. Hum Reprod. 2009;24(1):71-80.

[15] Huang da W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4(1):44-57.

[16] Moon SH, Kim SW, Kim JS, et al. Gene expression profiles in CHA3 and CHA4 human embryonic stem cells and embryoid bodies. Mol Cells. 2011;31(4):315-326.

[17] Zhao M, Ren CP, Yang H, et al. Shengming Kexue Yanjiu. 2008;12(1):61-65.赵明,任彩萍,杨红,等.人胚胎干细胞和拟胚体基因表达谱的初步分析[J].生命科学研究,2008,12(1):61-65.

[18] Rocha S. Gene regulation under low oxygen: holding your breath for transcription. Trends Biochem Sci. 2007;32(8): 389-397.

Effects of different oxygen tensions on gene expression profiling of human embryonic stem cells

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Jiang Tao, Ma Lei, Li Zhiqiang, Shou Xi, Duan Mingjun, Wu Shuo, Ma Chuang, Wei Qin. Platelet-derived growth factor BB induces bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(25): 3937-3942.
[2]	Mo Jianling, He Shaoru, Feng Bowen, Jian Minqiao, Zhang Xiaohui, Liu Caisheng, Liang Yijing, Liu Yumei, Chen Liang, Zhou Haiyu, Liu Yanhui. Forming prevascularized cell sheets and the expression of angiogenesis-related factors [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3479-3486.
[3]	Chen Lei, Zheng Rui, Jie Yongsheng, Qi Hui, Sun Lei, Shu Xiong. In vitro evaluation of adipose-derived stromal vascular fraction combined with osteochondral integrated scaffold [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3487-3492.
[4]	Wei Qin, Zhang Xue, Ma Lei, Li Zhiqiang, Shou Xi, Duan Mingjun, Wu Shuo, Jia Qiyu, Ma Chuang. Platelet-derived growth factor-BB induces the differentiation of rat bone marrow mesenchymal stem cells into osteoblasts [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 2953-2957.
[5]	Chen Xiao, Guo Zhi, Chen Lina, Liu Xuanyong, Zhang Yihuizhi, Li Xumian, Wang Yueqiao, Wei Liya, Xie Jing, Lin Li. Factors affecting the mobilization and collection of autologous peripheral blood hematopoietic stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 2958-2962.
[6]	Guo Zhibin, Wu Chunfang, Liu Zihong, Zhang Yuying, Chi Bojing, Wang Bao, Ma Chao, Zhang Guobin, Tian Faming. Simvastatin stimulates osteogenic differentiation of bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 2963-2968.
[7]	Li Congcong, Yao Nan, Huang Dane, Song Min, Peng Sha, Li Anan, Lu Chao, Liu Wengang. Identification and chondrogenic differentiation of human infrapatellar fat pad derived stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 2976-2981.
[8]	Gao Yuanhui, Xiang Yang, Cao Hui, Wang Shunlan, Zheng Linlin, He Haowei, Zhang Yingai, Zhang Shufang, Huang Denggao. Comparison of biological characteristics of adipose derived mesenchymal stem cells in Wuzhishan inbreed miniature pigs aged two different months [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 2988-2993.
[9]	Cao Yang, Zhang Junping, Peng Li, Ding Yi, Li Guanghui. Isolation and culture of rabbit aortic endothelial cells and biological characteristics [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3000-3003.
[10]	Dai Min, Wang Shuai, Zhang Nini, Huang Guilin, Yu Limei, Hu Xiaohua, Yi Jie, Yao Li, Zhang Ligang. Biological characteristics of hypoxic preconditioned human amniotic mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3004-3008.
[11]	Qin Yanchun, Rong Zhen, Jiang Ruiyuan, Fu Bin, Hong Xiaohua, Mo Chunmei. Chinese medicine compound preparation inhibits proliferation of CD133+ liver cancer stem cells and the expression of stemness transcription factors [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3016-3023.
[12]	Dai Yaling, Chen Lewen, He Xiaojun, Lin Huawei, Jia Weiwei, Chen Lidian, Tao Jing, Liu Weilin. Construction of miR-146b overexpression lentiviral vector and the effect on the proliferation of hippocampal neural stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3024-3030.
[13]	Chen Jie, Liao Chengcheng, Chen Zhiwei, Wang Yan. Bladder cancer stem cell markers and related signaling pathways: antibody targeted therapy [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3090-3096.
[14]	Zhang Wei, Cui Shuaishuai, Zhou Zhichao, Hu Xiaohua, Yang Xiaohong. MicroRNA regulates histone deacetylase in the treatment of bone-related diseases [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(17): 2767-2774.
[15]	Chen Liang, Meng Shu, Cheng Guoping, Ding Yi . Effects of fish scale collagen membrane on adhesion, proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2494-2499.