Bone morphogenetic protein 2 co-transfected with bone morphogenetic protein 7 forosteogenic differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2012.49.004

Abstract

Abstract:

BACKGROUND: The most important role of bone morphogenetic protein is the induction of bone formation some factors, including difficulty in sample extract, rapid metabolic rate, difficulty in accurate concentration control and high cost, limit the application for in vitro and in vivo studies.
OBJECTIVE: To establish the adenoviral vectors containing bone morphogenetic protein 2 and bone morphogenetic protein 7 in order to observe the promotion effect of co-transfection of bone morphogenetic protein 2 and bone morphogenetic protein
METHODS: The rabbit bone marrow mesenchymal stem cells separated by the whole bone marrow adherence method were passaged to the third generation, and divided into five groups: the control group and the conventional induction group were cultured with conventional culture medium and osteo-induction culture medium; bone morphogenetic protein 2 group and bone morphogenetic protein 7 group were transfected with bone morphogenetic protein 2 adenovirus and bone morphogenetic protein 7 adenovirus; the combination group was co-transfected with bone morphogenetic protein 2 adenovirus and bone morphogenetic protein 7 adenovirus.
RESULTS AND CONCLUSION: At 7 days after transfection, the expression levels of Runx-2, Osx, alkaline phosphatase and Collagen Ⅰ mRNA in the combination group were higher than those in the other four groups (P < 0.05); the indicators above in the bone morphogenetic protein 2 group and bone morphogenetic protein 7 group were higher than those in control group and the conventional induction group (P < 0.05). At 14 days after transfection, the expression of Runx-2, Osx,alkaline phosphatase and collagen Ⅰ mRNA in the combination group were higher than those in the other four groups (P < 0.05); and the indicators at 14 days after transfection were higher than those at 7 days after trasnfection (P < 0.05). At 7 days after transfection, the expression levels of collagen Ⅰ and osteocalcin protein in the combination group were higher than those in the other four groups (P < 0.05). After co-transfected by both bone morphogenetic protein 2 and bone morphogenetic protein 7 adenoviral vectors, bone marrow mesenchymal stem cells could express high levels of osteogenesis related gene in both mRNA level and protein level than induced by conventional culture medium or osteo-induction culture medium.

CLC Number:

R394.2

Chen Jian, Yuan Wen, Song Dian-wen, Hu Kai-meng, Fan Li-xing, Liu Hou-qi. Bone morphogenetic protein 2 co-transfected with bone morphogenetic protein 7 forosteogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9139-9145.

Figures/Tables 6

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