Mesenchymal stem cells transformed to myogenic cell induced by 5-azacytidine in vitro

doi:10.3969/j.issn.2095-4344.2012.49.003

Abstract

Abstract:

BACKGROUND: With the characteristics of multiple differentiation potential and the in vitro expression of a variety of exogenous genes, bone marrow mesenchymal stem cells are used as the seed cells for the future repairing and replacing of tissues and organs and the research has become the hot spot. To date, bone marrow mesenchymal stem cells have been isolated from human and several kinds of animals, but reports on porcine bone marrow mesenchymal stem cells are rare.
OBJECTIVE: To establish a method for culturing porcine bone marrow mesenchymal stem cells and then transformation into myogenic cells in vitro, and to investigate the underlying biological mechanisms.
METHODS: Porcine bone marrow mesenchymal stem cells were isolated and purified by centrifugation. After induced with 5-azacytidine, the immunohistochemical staining was performed with desmin, MHC, cTnI and Cx-43.
RESULTS AND CONCLUSION: After induced with 5-azacytidine, some porcine bone marrow mesenchymal stem cells became spindle-like and were positive for desmin, MHC, cTnI and Cx-43 expression. Porcine bone marrow mesenchymal stem cells can be transformed into myogenic cells after in vitro induced with 5-azacytidine.

CLC Number:

R394.2

Zhou Ya-feng, Li Hong-xia, Cheng Cheng, Han Lian-hua, Yang Xiang-jun. Mesenchymal stem cells transformed to myogenic cell induced by 5-azacytidine in vitro[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9134-9138.

Figures/Tables 2

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