Differentiation of rabbit bone marrow mesenchymal stem cells into hepatocyte-like cells in vitro

doi:10.3969/j.issn.2095-4344.2012.49.002

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells transplantation can significantly improve liver function and promote liver regeneration in a mouse model of severe liver failure induced by carbon tetrachloride.
OBJECTIVE: To investigate liver function and hepatic cirrhosis degree after bone marrow mesenchymal stem cells transplantation.
METHODS: Bone marrow mesenchymal stem cells were isolated by density gradient centrifugation method and adherent culture method. Passage 3 bone marrow mesenchymal stem cells were treated with hepatocyte growth factor and epidermal growth factor and labeled with BrdU after induced for 21 days. At the same time, animal models of liver cirrhosis were established. The BrdU-labeled hepatocyte-like cells suspension and normal saline were injected into rabbits in the experimental and control groups through the portal vein. The rabbits were sacrificed under anesthesia at 21 days after transplantation. The hepatic cirrhosis degree and liver function of rabbits with cirrhosis were investigated.
RESULTS AND CONCLUSION: Morphological changes in bone marrow mesenchymal stem cells were observed after in vitro induced with hepatocyte growth factor and epidermal growth factor for 21 days and cell morphology changed into round. Cell staining results showed positive expression of alpha-fetoprotein, serum albumin and glycogen. After transplantation of hepatocyte-like cells through the portal vein, the liver biochemical indicators in the experimental group were significantly improved when compared with the control group (P < 0. 05). BrdU immunohistochemical staining showed the BrdU-positive cells were observed in the portal area and hepatic sinusoids. Rabbit bone marrow mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells, and the induced cells can improve rabbit liver function and promote hepatocyte regeneration.

CLC Number:

R394.2

Li De-qiang, Wang Ren-hao. Differentiation of rabbit bone marrow mesenchymal stem cells into hepatocyte-like cells in vitro [J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9128-9133.

Figures/Tables 13

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