Trans-differentiation of bone marrow mesenchymal stem cells into functional neurons

doi:10.3969/j.issn.2095-4344.2012.49.001

Abstract

Abstract:

BACKGROUND: It has the important theoretical and practical significance to induce the differentiation of bone marrow mesenchymal stem cells into neural stem cells and to investigate the method to improve the differentiation efficiency.
OBJECTIVE: To investigate the method of trans-differentiation of bone mesenchymal stem cells into functional neurons.
METHODS: Bone marrow mesenchymal stem cells were isolated and purified from rat bone marrow by density gradient centrifugation and adherent culture. Expression of bone marrow mesenchymal stem cells surface marker was detected by flow cytometry. All bone marrow mesenchymal stem cells were divided into three groups: neurotrophic factor induction group, chemical induction group and control group. The bone marrow mesenchymal stem cells in the neurotrophic factor induction group were induced with brain-derived neurotrophic factor and basic fibroblast growth factor, the cells in the chemical induction group were induced with dimethyl sulfoxide and butylated hydrochloride, and the cells in the control group were induced with PBS.
RESULTS AND CONCLUSION:The bone marrow mesenchymal stem cells in the induction groups could express neuron specific enolase and microtubule-associated protein-2, and there was no significant difference of the expression rate between these two groups (P > 0.05), while in the control group, no expression of neuron specific enolase and microtubule-associated protein-2 was detected. Patch clamp system detection showed that the induced bone marrow mesenchymal stem cells in the neurotrophic factor induction group could express action potential with the characteristics of neural cells and excitatory postsynaptic currents. There were no electrophysiologica characteristics in the chemical induction group and control group. It indicates that brain-derived neurotrophic factor combined with basic fibroblast growth factor is an effective method of inducing bone marrow mesenchymal stem cells to trans-differentiate into functional neurons.

CLC Number:

R394.2

Huang Bao-sheng, Wang Tian-lu, Li Li-xin, Xie Qing-song, Tian He-ping, Zhang Yin. Trans-differentiation of bone marrow mesenchymal stem cells into functional neurons[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9121-9127.

Figures/Tables 5

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