Effects of red light-emitting diodes with different light energies on proliferation of human periodontal ligament stem cells and apical papillary stem cells

doi:10.3969/j.issn.2095-4344.1744

Abstract

Abstract:

BACKGROUND: Photobiomodulation is a new treatment option. Whether red light-emitting diodes can affect the proliferation and regeneration of dental stem cells is the key to exploring new treatments for maxillofacial regeneration.
OBJECTIVE: To compare the effects of red light-emitting diodes with different light energies on the proliferation of human periodontal ligament stem cells and apical papillary stem cells.
METHODS: Periodontal ligament stem cells and apical papillary stem cells were isolated and cultured by flow cytometry. Proliferation of these cells was detected using MTT assay after exposure to red light-emitting diodes at different light energies (0, 1, 3, and 5 J/cm²).
RESULTS AND CONCLUSION: Apical papillary stem cells proliferated faster than periodontal ligament stem cells under non-lighting environment. Red light emitting diodes at 1, 3, and 5 J/cm² promoted the proliferation of two kinds of odontogenic stem cells. The red light-emitting diode promoted the proliferation of periodontal ligament stem cells at proliferative and logarithmic growth phase (1-7 days). And 5 J/cm2 red light emitting diode had the most obvious effect in early stage, and 1 J/cm² had the strongest effect in the late stage. The red light-emitting diode had a significant effect on the logarithmic growth (5-7 days) of apical papilla stem cells, and under 3 J/cm² red light-emitting diode the proliferation peaked on the 7th day. Therefore, the red light-emitting diode can promote the proliferation of human periodontal ligament stem cells and apical papillary stem cells in dose- and time-dependent manner. Exposure to red light-emitting diode at proper energy and time can promote cell proliferation quickly and effectively.

Key words: apical papillary stem cells, periodontal ligament stem cells, odontogenic stem cells, red light-emitting diode, cell proliferation, light energy, photobiomodulation

CLC Number:

R459.9

Wu Yan, Yang Yaoyao, Wang Yao. Effects of red light-emitting diodes with different light energies on proliferation of human periodontal ligament stem cells and apical papillary stem cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(21): 3289-3293.

Figures/Tables 3

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