Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3929-3932.doi: 10.3969/j.issn.1673-8225.2012.21.030

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High efficient nerve growth factor made by double emulsion water/oil/water method

Gu Hai-gang1, Li Xiao-bin1, Long Da-hong2   

  1. 1Department of Human Histology and Embryology, 2Department of Human Anatomy, Guangzhou Medical College, Guangzhou  510182, Guangdong Province, China
  • Online:2012-05-20 Published:2012-05-20
  • About author:Gu Hai-gang☆, Doctor, Lecturer, Department of Histology and Embryology, Guangzhou Medical College, Guangzhou 510182, Guangdong Province, China guhaigang@ yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No.30800345*; Medical Scientific Research Foundation of Guangdong Province, No.B2008071*; Foundation from Guangzhou Medical College, No.0706059*

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Abstract:

BACKGROUND: Nerve growth factor (NGF) is a macromolecular protein that cannot easily cross the blood-brain barrier. Gene therapy can successfully deliver neurotrophic factors into the brain.
OBJECTIVE: To explore how to formulate the high efficient NGF gene nanoparticles.
METHODS: NGF nanoparticles were prepared by double emulsion water/oil/water method. Chitosan and Tat polypeptide were used to modify NGF nanoparticles. Neuron degeneration of cholinergic neurons in the rat’s basal forebrain was induced by fimbria-fornix transection. Sixteen SD rats were randomly divided into injury group and nanoparticles group micro injected with NGF gene in the basal forebrain. 
RESULTS AND CONCLUSION: The distribution of particle size of NGF nanoparticles was from 110 nm to 150 nm. Chitosan could increase zeta potential and loading efficiency of NGF nanoparticles, but not particle size. Tat polypeptide could significantly increase the transfection efficiency of NGF nanoparticles. It indicated that water/oil/water method could successfully make the NGF nanoparticles. And the NGF nanoparticles transplantation could protect the basal forebrain.

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