Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3915-3918.doi: 10.3969/j.issn.1673-8225.2012.21.027

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Effects of four kinds of base metal alloy crown extract on expression levels of urokinase-type plasminogen activator and plasminogen activator inhibitor-1 in human gingival fibroblasts 

Lu Lin, Huang Ke-qiang, Hu Jing, Du Ya   

  1. Department of Orthodontics, Second Affiliated Hospital of Liaoning Medical University, Jinzhou  121004, Liaoning Province, China
  • Received:2012-01-29 Revised:2012-03-17 Online:2012-05-20 Published:2012-05-20
  • Contact: Huang Ke-qiang, Master, Professor, Department of Orthodontics, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121004, Liaoning Province, China hkq9@163.com
  • About author:Lu Lin★, Studying for master’s degree, Department of Orthodontics, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121004, Liaoning Province, China maomaoyijia@126.com

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Abstract:

BACKGROUND: Biocompatibility of repairing materials is an important factor for clinical effects.
OBJECTIVE: To evaluate the material biocompatibility in oral repair by analyzing different expressional levels of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) of human gingival fibroblasts (HGFs) in four types of metal extractions.
METHODS: Healthy gums were obtained to cultivate HGFs. Levels of PAI-1 and uPA were examined by immunofluorescence and enzyme linked immunosorbent assay (ELISA) detection after different procedures: Co-Cr, Ni-Cr, Ti and rhotanium metal extractions. HGFs with no treatment served as controls.
RESULTS AND CONCLUSION: ELISA results showed an increase in uPA levels after treatment with Co-Cr and Ni-Cr metal extractions. Immunofluorescence detection and electron microscope revealed deep-stained and even-distributed cytoplasm after treatment with Co-Cr and Ni-Cr, indicating PAI-1 levels were increased. There were changes in the levels of uPA and PAI-1 after treatment with Ti and gold alloy metal extractions. Biocompatibility of Ti and rhotanium is better than that of Co-Cr and Ni-Cr.

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