Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3895-3898.doi: 10.3969/j.issn.1673-8225.2012.21.022

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In vitro co-culture of injectable alginate scaffolds and human bone marrow stromal stem cells

Wang Qun-li1, Gong Ji-cheng1, Zou Zhong-wen1, Yang Bo-lin1, Luo Wei-guo1, Jin Dan2, Wei Kuan-hai2   

  1. 1Second Department of Orthopedics, the 187 Central Hospital of Chinese PLA, Haikou  571159, Hainan Province, China; 2Department of TraumaticOrthopedics, Nanfang Hospital of Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2012-01-09 Revised:2012-02-21 Online:2012-05-20 Published:2012-05-20
  • About author:Wang Qun-li☆, Doctor, Associate chief physician, Second Department of Orthopedics, the 187 Central Hospital of Chinese PLA, Haikou 571159, Hainan Province, China Wangqunli2005@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Hainan Province, No. 808248*

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Abstract:

BACKGROUND: Injectable tissue-engineered bone is limited to animal experiments. If the human bone marrow stromal stem cells have a good biocompatibility with alginate, the injectable tissue engineered bone will be a very promising clinical treatment.
OBJECTIVE: To study the biocompatibility of human bone marrow stromal stem cells and injectable scaffold of calcium alginate gels in vitro.
METHODS: The second passage human bone marrow stromal stem cells were co-cultured with calcium alginate gels. Bone marrow stromal stem cells cultured with no material served as controls. The morphology and proliferation of the cells were observed by inverted phase contrast microscope and scanning electron microscope. 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay was used to semi-quantitatively evaluate the cell proliferation.
RESULTS AND CONCLUSION: The human bone marrow stromal stem cells co-cultured with calcium alginate gels grew well under inverted phase contrast microscope, and no significant difference was found between calcium alginate gels group and control group. Under scanning electron microscope, human bone marrow stromal stem cells adhered well to the calcium alginate gels and proliferated obviously. MTT results showed that the cell number had no significant difference between the two groups. It is suggested that calcium alginate gels have good biocompatibility with human bone marrow stromal stem cells.

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