Abstract:

BACKGROUND: Diamond-like carbon film of pulsed laser method deposition and the low temperature isotropic carbon are considered as amorphous carbon. They are presented with good physical characteristics and biocompatibility, simple operation, and have become the selectable material for constructing tissue engineering valves at present.
OBJECTIVE: To observe the biocompatibility of primary canine vein endothelial cells and diamond-like carbon valves prepared by pulsed laser deposition method.
METHODS: Venous vascular endothelial cells from the forelimb of hybrid canines were isolated, and co-cultured with diamond-like carbon nano-coating valves prepared by pulsed laser deposition, serving as the experimental group. Pure canines forelimb vein vascular endothelial cells were as control group.
RESULTS AND CONCLUSION: Nitric oxide secretory function of the two groups has no difference. Vascular endothelial cells around diamond-like carbon film material were observed with the inverted microscope, and there was no aging and abnormal mitotic phenomenon. Scanning electron microscopy observation showed that vascular endothelial cells adhered on diamond-like carbon film closely; besides, cells were extended and interconnected with each other. Some cells were integrated together and diamond-like carbon film was almost covered. These findings suggest that diamond-like carbon nano-coating film has well biocompatibility.