Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3819-3822.doi: 10.3969/j.issn.1673-8225.2012.21.005

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Repairing bone defects by non-cell based tissue engineered complex constructed by autologous red bone marrow

Huang Wen-liang1, Deng Jiang1, Jia Dong-lin2, Ruan Shi-qiang1, Yuan Cheng1   

  1. 1Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China; 2Department of Orthopedics, First People’s Hospital of Yanzhou City, Yanzhou  272116, Shandong Province, China
  • Received:2012-01-17 Revised:2012-03-13 Online:2012-05-20 Published:2012-05-20
  • Contact: Deng Jiang, Chief physician, Master’s supervisor, Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China DJ30666@126.com
  • About author:Huang Wen-liang★, Master, Physician, Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the Science and Technology Foundation of Zunyi City, No. [2007]15*; the Social Tackle Key Program of Guizhou Province, No. [2010]3101*

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Abstract:

BACKGROUND: Cell based tissue engineered bone is successful in the repair of bone defects, but it is a complex operation, easy to be contaminated and takes a long time, and not conducive to clinical application.
OBJECTIVE: To investigate the efficacy of repairing large-segment bone defects by non-cell based tissue engineered complex constructed by autologous red bone marrow.
METHODS: Twenty-seven rabbits were used to prepare 2 cm unilateral radial large-segment bone defect models, and then randomly divided into group A, group B and group C. Group A was implanted with autologous red bone marrow to construct tissue engineered bone (autologous red bone marrow+recombinant human insulin-like growth factor-1/coralline hydroxyapatite); group B was implanted with autologous red bone marrow/coralline hydroxyapatite artificial bone material; group C was implanted with coralline hydroxyapatite/recombinant human insulin-like growth factor-1 artificial bone material.
RESULTS AND CONCLUSION: Group A was superior to the other two group in terms of osteogenic potential and material degradation by comparison of degree of bone formation, bone trabecular number, the structure formation of mature bone and implant degradation at 4, 8, 12 weeks after implantation (P < 0.05). Non-cell based tissue engineered bone constructed by autologous red bone marrow has better osteogenic activity, and effective to repair large-segment bone defects.

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