Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3811-3814.doi: 10.3969/j.issn.1673-8225.2012.21.003

Previous Articles     Next Articles

Preparation and in vitro degradation of poly(hydroxybutyrate-co-hydroxyoctanoate)/acellular cartilage matrix composite scaffolds  

Zhang Peng, Zhang Yong-hong   

  1. Department of Orthopedic Trauma, Second Affiliated Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2011-12-07 Revised:2012-02-03 Online:2012-05-20 Published:2012-05-20
  • About author:Zhang Peng★, Studying for master’s degree, Department of Orthopedic Trauma, Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China sophyzp@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 31040030*

PDF

442

Abstract:

BACKGROUND: Poly (hydroxybutyrate-co-hydroxyoctanoate) (PHBHOx) has good biological characteristics, high biocompatibility and biodegradability. But the single material cannot satisfy the requirements of tissue engineering composite scaffolds. Acellular cartilag matrix (ACM) is commonly used in the preparation of composite scaffolds, and has excellent biocompatibility and no antigenicity.
OBJECTIVE: To prepare the composite scaffolds and to observe the degradation rate of PHBHOx/ACM in vitro.
METHODS: The articular cartilage from pigs were immersed in phosphate buffered saline containing double antioxidant, then cultured in tris hydroxymethyl aminomethane containing phenylmethanesulfony fluoride, digested with deoxyribonuclease and ribonuclease and rinsed with D-Hank’s solution to prepare ACM samples. Solvent casting-particle leaching method was used to prepare composite scaffolds with different ratios of ACM and PHBHOx.
RESULTS AND CONCLUSION: In different proportion of PHBHOx/ACM composite scaffolds, 8% ACM accords with requirements for cartilage tissue engineering scaffolds.
 

CLC Number: