Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3801-3805.doi: 10.3969/j.issn.1673-8225.2012.21.001

    Next Articles

Histological observation of dog peripheral nerve regeneration induced by a novel biomimetic nerve conduit

Xiang Jie1,2, Huang Ji-feng2, Li De-zhong2, Li Shi-pu3, Yan Yu-hua3, Yan Qiong-jiao3   

  1. 1Southern Medical University, Guangzhou  510515, Guangdong Province, China; 2Department of Orthopedics, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan  430070, Hubei Province, China; 3Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan  430070, Hubei Province, China
  • Received:2012-02-27 Revised:2012-03-14 Online:2012-05-20 Published:2012-05-20
  • Contact: Huang Ji-feng, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan 430070, Hubei Province, China huangjfgk@ yahoo.com.cn
  • About author:Xiang Jie★, Studying for master’s degree, Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Orthopedics, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan 430070, Hubei Province, China xiangjiegk@163.com
  • Supported by:

     Hubei Natural Science Priority Research Fund, No. 2009CDA041*; Wuhan Science and Technology Research Program, No. 200960323125*

PDF

517

Abstract:

BACKGROUND: Previous research has shown that the novel arginine-glycine-aspatic acid (RGD) peptide modification of poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]}/poly(d,l-lactic acid)/β-tricalcium phosphate/nerve growth factor (PRGD/PDLLA/β-TCP/NGF) composite nerve conduit can effectively repair 10-mm sciatic nerve defects of rats, while it possess good cell affinity and biocompatibility.
OBJECTIVE: To observe the histological observation of dog peripheral nerve regeneration with PRGD/PDLLA/β-TCP/NGF composite nerve conduit.
METHODS: Twelve healthy hybrid dogs were used as animal models. The conduits were employed to bridge the 30 mm defects in the peroneal nerve.
RESULTS AND CONCLUSION: Newborn regenerated myelinated nerve fibers were found in the distal section at 3 months. The diameters of myelinated nerve fibers and thickness of myelin sheath were increased day by day. Tibialis anterior muscles showed gradual atrophy after operation, which peaked 3 months after denervation. Subsequently, the atrophy of tibialis anterior muscles recovered slowly at 6 and 9 months. This study proves the feasibility of the PRGD/PDLLA/β-TCP/NGF nerve conduit for repairing large-diameter nerve defects in large animals.

CLC Number: