Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3730-3734.doi: 10.3969/j.issn.1673-8225.2012.20.027

Previous Articles     Next Articles

Effect of aerobic exercise on the expression of microRNA-133a and myocyte enhancer factor 2 in the gastrocnemius

Zhang Jia-wei1, Ren Wen-jun2   

  1. 1Department of Physical Education, Xi'an Financial Services Institute, Xi’an  710100, Shaanxi Province, China; 2Sports Center, Xi’an Jiaotong University, Xi’an  710049, Shaanxi Province, China
  • Received:2012-01-05 Revised:2012-03-19 Online:2012-05-13 Published:2012-05-13
  • About author:Zhang Jia-wei★, Master, Lecturer, Department of Physical Education, Xi'an Financial Services Institute, Xi’an 710100, Shaanxi Province, China xazhangjiawei@163.com
  • Supported by:

    the International Cooperation Item of Shaanxi Province, No. 2010kw-05*; the Science and Technology Program of Xi’an City, No. cxy1128*; the Fundamental Research Funds for the Central Universities, No. sk2011041*

PDF

302

Abstract:

BACKGROUND: Studies have demonstrated that mircoRNA-133a, myocyte enhancer factor 2, and myogenic differentiation antigen can regulate the development and remodeling of skeletal muscle.
OBJECTIVE: To investigate the effect of aerobic exercise on the expression of mircoRNA-133a, myocyte enhancer factor 2, and myogenic differentiation antigen in the gastrocnemius.
METHODS: Forty male SD rats were randomized into control and training group. The treadmill training was performed in the training group, and no training was in the control group. After training for 4 and 6 weeks, myosin, microRNA-133a, myocyte enhancer factor 2, myoblast differentiation antigen mRNA expressions in the gastrocnemius were detected using quantitative real-time PCR, and the changes in cross-section area of type Ⅱ muscle fiber was detected using immunohistochemistry method.
RESULTS AND CONCLUSION: After the continuous treadmill training for 4 and 6 weeks, the relative weight of gastrocnemius and the contents of myosin heavy chain II a mRNAs increased significantly compared with the control group (P < 0.05 or P < 0.01). Simultaneously, the cross-sectional area of type Ⅱ muscle fibers increased (P﹤0.05). The expressions of mircoRNA-133a and myocyte enhancer factor 2 increased significantly (P < 0.05 and P < 0.01) in training groups after 4 and 6 weeks of aerobic exercise, while the expression of myogenic differentiation antigen maintained the same levels. It is indicated that aerobic exercise elevates the expressional levels of mircoRNA-133a and myocyte enhancer factor 2.
 

CLC Number: