Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3725-3729.doi: 10.3969/j.issn.1673-8225.2012.20.026

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Shudi Biejia and mesangial cells cooperatively up-regulate the gene and protein expression of osteocalcin in osteoblasts

Lin Ri-yang1, Wu Feng1, 2, He Li-qun1, 2, 3   

  1. 1Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai  200021, China; 2Key Laboratory of Clinical Study for Traditional Chinese Medicine of Shanghai, Shanghai  201203, China; 3the Ministry of Education Key Laboratory of Liver and Kidney Diseases, E-Institute of Chinese Internal Medicine of Shanghai Colleges and Universities, Shanghai  200021, China
  • Received:2011-09-20 Revised:2011-10-24 Online:2012-05-13 Published:2012-05-13
  • Contact: He Li-qun, Doctor, Chief physician, Doctoral supervisor, Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China; Key Laboratory of clinical Study for Traditional Chinese Medicine of Shanghai, Shanghai 201203, China; the Ministry of Education Key Laboratory of Liver and Kidney Diseases, E-Institute of Chinese Internal Medicine of Shanghai Colleges and Universities, Shanghai 200021, China
  • About author:Lin Ri-yang☆, Studying for doctorate, Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China lin_ri_yang@126.com
  • Supported by:

    the Key Basic Research Project of Shanghai Committee of Science and Technology, No. 09JC1413700*; the Innovation Team of Shanghai Municipal Education Commission*

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Abstract:

BACKGROUND: Serum containing Shudi and Biejia up-regulates the gene expression of osteocalcin in osteoblasts; the mesangial cells supernatant can also up-regulate the gene expression of osteocalcin in osteoblasts.
OBJECTIVE: To detect if there are additive effects of the mesangial cells supernatant and serum containing Shudi and Biejia on the gene and protein expression of osteocalcin in osteoblasts.
METHODS: Primary cultured osteoblasts of SD rats were divided into four groups after identification. Control group contained blank rat serum. Shudi Biejia group contained rat serum containing Shudi Biejia. Mesangial cell supernatant group contained blank rat serum and mesangial cell supernatant. Shudi Biejia plus mesangial cell supernatant group contained mesangial cell supernatant and rat serum containing Shudi Biejia. Culture medium was collected on day 6 after cultivation and was well mixed with the culture medium collected on day 3 after cultivation for detection.
RESULTS AND CONCLUSION: Compared with the control group, the mRNA expression of osteocalcin in Shudi Biejia group, mesangial cell supernatant group and Shudi Biejia plus mesangial cell supernatant group was significantly up-regulated (P < 0.05); the expression rate of osteocalcin mRNA in the Shudi Biejia plus mesangial cell supernatant group was significantly higher than that in the Shudi Biejia group and mesangial cell supernatant group. The absorbance value of osteoblastic osteocalcin concentration in the Shudi Biejia group and mesangial cell supernatant group was significantly higher than that in the control group (P < 0.05). Compared with Shudi Biejia group, the absorbance value of osteoblastic osteocalcin concentration in the Shudi Biejia plus mesangial cell supernatant group was significantly increased (P < 0.05). These findings indicate that the mesangial cell supernatant and serum containing Shudi Biejia both can increase the gene expression of osteocalcin in osteoblast; the combined action of the two can further up-regulate the gene expression of osteocalcin; meanwhile the osteocalcin secretion also increases significantly.
 

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