Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3639-3643.doi: 10.3969/j.issn.1673-8225.2012.20.007

Previous Articles     Next Articles

Effect of stromal cell derived factor-1 on collagen type Ⅱ and matrix metalloproteinase secretion in osteoarthritis

Wang Guo-liang, Li Yan-lin, Gao Gang, Ma Ke, Chen Wen-dong, Xu Peng, Yang Guang   

  1. Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming  650031, Yunan Province, China
  • Received:2011-10-16 Revised:2011-11-12 Online:2012-05-13 Published:2012-05-13
  • Contact: Li Yan-lin, Professor, Doctoral supervisor, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunan Province, China yanlinli1969@yahoo.com.cn
  • About author:Wang Guo-liang★, Studying for master’s degree, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunan Province, China 200301144@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860286*

PDF

315

Abstract:

BACKGROUND: The stromal cell derived factor-1 (SDF-1)/chemokine receptor 4 (CXCR4) signal pathway plays a key role in the pathogenesis of osteoarthritis.
OBJECTIVE: To study the effect of SDF-1 on collagen type Ⅱ and matrix metalloproteinase (MMPs) secretion in osteoarthritis.
METHODS: Forty knee cartilage blocks from osteoarthritis patients who had total knee replacement were selected as experimental group. Forty knee cartilage blocks from patients who had traumatic amputation were as the control group. The samples were put in Dulbecco's modified Eagle’s medium and cultivated for 48 and 96 hours with 79 and 392 μg/L exogenous SDF-1.
RESULTS AND CONCLUSION: After cultured for 48 and 96 hours, the expressions of collagen type Ⅱ were both positive in the two groups by immunohistochemistry staining; the collagen type Ⅱ degenerated more when it was exposed in high-concentration SDF-1 culture medium with the longer time. There was no significant difference of SDF-1 in SDF-1 culture medium at the same time and concentration between the experimental and control groups (P﹥0.05). The MMPs of the experimental group was released more than that of the control group in high-concentration SDF-1 culture medium (P < 0.05). These showed that SDF-1 could regulate the expression of MMPs and lead to the degradation of collagen type Ⅱ through SDF-1/CXCR4 signal pathways in osteoarthritis. 

CLC Number: