Abstract:

BACKGROUND: The specific markers in joints, blood, urine and other body fluids that can reflect the degree of articular cartilage damage can be detected by proteomics. 
OBJECTIVE: To further verify the application of biological chip technology to find rabbit articular cartilage damage biological markers.
METHODS: The improved Hulth method was used to establish the rabbit articular cartilage damage model. Postoperative free activities and the injury limb were not fixed, 30 min/d points off twice for 12 weeks. The rabbit knee without any treatment was as normal control. At 4, 8 and 12 weeks after modeling, part of the specimens (such as serum and joint liquid) were collected, and the articular cartilage damage degree was observed and verified; the joint fluid and serum sample of the animals at each time points were put into the PBS Ⅱ-C protein microarray reading machine and detected with CM10 chip.
RESULTS AND CONCLUSION: The articular cartilage damage model established by improved Hulth method could quite comprehensively reflect the change of articular cartilage damage from the early, middle, and late and decompensate periods. Compared with normal control group, the expression of 3 475 protein in serum specimen was decreased and the expression of    7 558, 15 475 and 33 665 protein was increased in model group; the expression of 7 558 and 33 278 protein in joint fluid specimen was decreased and the expression of 3950 and 16 055 protein was increased in model group. The significant differences protein peak spectrum with the nuclear mass ratio of 3 475, 7 558, 16 884 was expressed in both serum specimen and joint fluid specimen. Part of the differential protein appeared in the specimen may be the articular cartilage damage biological markers.