Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3471-3475.doi: 10.3969/j.issn.1673-8225.2012.19.011

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Umbilical cord blood mesenchymal stem cells transplantation for the treatment of cerebral ischemia injury in rats

Liu Hui-chun1, Zhang Pei-pei1, Wang Shi-min2, Yan Xiao-ling3, Wang Xin-ping2, Zhang Wen-zhi4, Tang Fan3   

  1. 1Tianjin Medical University, Tianjin  300070, China; 2Department of Neurology, 3Departement of Pathology, 4Cell Room of Neurosurgery Institute, Huanhu Hospital of Tianjin, Tianjin  300060, China
  • Received:2011-08-26 Revised:2011-10-24 Online:2012-05-06 Published:2012-05-06
  • Contact: Wang Shi-min, Professor, Master’s supervisor, Department of Neurology, Huanhu Hospital of Tianjin, Tianjin 300060, China wangxinpingtj@sina.com
  • About author:Liu Hui-chun★, Studying for master’s degree, Tianjin Medical University, Tianjin 300070, China liuhuichungood@163.com
  • Supported by:

    Major Science and Technology Support Planning Project of Tianjin, China, No. 08ZCKF03200*

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Abstract:

BACKGROUND: There is yet no medicine that is confirmed to improve ischemic stroke. The high self-renewal ability and multiplex differentiation potential make it possible for stem cells to rebuild the structure and function of the central nervous system (CNS).
OBJECTIVE: To observe the effect and safety of umbilical cord blood mesenchymal stem cells transplantation for the treatment of ischemic stroke in rat models and to investigate its possible mechanism.
METHODS：Human umbilical cord blood mesenchymal stem cells were isolated in vitro by using density gradient centrifugation combined with adherent screening and labeled by BrdU before transplantation. The male SD rats were used to establish the middle cerebral artery occlusion model and then the models were randomly divided into 3 groups. In the transplantation group, the umbilical cord blood mesenchymal stem cells were transplanted into corpus striatum on the injury side at 7 days after modeling; the PBS group was given the same dose of PBS and the rats in model group were without treatment.
RESULTS AND CONCLUSION: The recovery of mNSS score of transplantation group was better than that of model group (P < 0.05), and the recovery of mNSS score in PBS group was slower than that in model group within 35 days (P < 0.05), and had no significant difference at the 56th day (P > 0.05). BrdU staining positive cells and Nestin, microtubule rapsyn 2, glial fibrillary acidic protein, Ⅷ factor and vascular endothelial growth factor immunohistochemical double staining positive cells could be seen in the center and surroundings of the injury area in transplantation group. It indicated that umbilical cord blood mesenchymal stem cells could be isolated in vitro, and could survive and differentiate in rat host brain and promote the recovery of the neural function of middle cerebral artery occlusion model rats. The mechanism might be related to the transplanted cells differentiating into neuron-like and neural glial-like cells, and secreting neurotrophic factors and promoting angiogenesis of host cells.

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