Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3438-3441.doi: 10.3969/j.issn.1673-8225.2012.19.004

Previous Articles     Next Articles

Co-cultivation of bone marrow stromal cells with rabbit osteoblast-like cells in a Transwell chamber for osteogenic differentiation 

Zhang Shu-ying1, Duan Da-bo2, Zhang Li2, Wang Zhi-ying1   

  1. 1Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China; 2Jinzhou Central Hospital, Jinzhou 121000, Liaoning Province, China
  • Received:2012-01-23 Revised:2012-02-18 Online:2012-05-06 Published:2012-05-06
  • Contact: Wang Zhi-ying, M.D., Professor, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China bdwzy@126.com
  • About author:Zhang Shu-ying★, Studying for master’s degree, Attending physician, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China 342797171@qq.com

PDF

450

Abstract:

BACKGROUND: Osteogenic differentiation is previously limited by culture of dexamethasone, growth factors or osteoblast-like cells with bone marrow mesenchymal stem cells at 1:1.
OBJECTIVE: To investigate the feasibility of in vitro co-cultivation of osteoblast-ike cells and bone marrow stromal cells (BMSCs) and osteoblast differentiation of BMSCs by osteoblast-like cells.
METHODS: Passage 3 rabbit osteoblast-like cells and passage 3 rabbit BMSCs were co-cultured in Transwell chamber, and osteoblast-like cells were inoculated into the lower part of culture plate. BMSCs were inoculated on Transwell inner membrane, serving as experimental group. BMSCs were inoculated into Transwell chamber alone with basal medium in the lower part, serving as control group.
RESULTS AND CONCLUSION: In the experimental group, BMSCs differentiated toward osteoblasts, and alkaline phosphatase activity was significantly higher compared with the control group (P < 0.05). In the experimental group, BMSCs were positive for alizarin red staining, showing red calcified nodules; after RT-PCR, the expression of core-binding factor α1 was observed. In the control group, calcified nodules were not observed. These findings suggest that Transwell chamber can help to achieve the in vitro co-cultivation of osteoblast-like cells and BMSCs and the osteogenic differentiation of BMSCs.

CLC Number: