Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (18): 3263-3266.doi: 10.3969/j.issn.1673-8225.2012.18.008

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Effects of hormone on heart function protection and the levels of serum calcitonin-gene-related peptide and endothelin-1 in brain-dead pigs

Wang Xi, Ma Li-ming, Jia Shao-jun, Yang Shao-jun   

  1. Department of Thoracic and Cadiovascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming  650101, Yunnan Province, China
  • Received:2011-12-24 Revised:2012-01-24 Online:2012-04-29 Published:2012-04-29
  • Contact: Yang Shao-jun, Chief physician, Master’s supervisor, Department of Thoracic and Cadiovascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China yangsj8@hotmail. com
  • About author:Wang Xi, Attending physician, Department of Thoracic and Cadiovascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China wangxi1a2b@sina. com
  • Supported by:

    Joint Special Projects of Science and Technology Department of Yunnan Province-Kunming Medical College, No.2008CD026*; Health Science and Technology Projects Fund of Yunnan Province, No.2009NS076*

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Abstract:

BACKGROUND: Hormones can reduce the heart ischemia-reperfusion injury of brain-dead donor and reduce the release of inflammatory factors, and play a role in the protection of the donor heart after brain death. 
OBJECTIVE: To investigate the effects of hormone on heart function of brain-dead pigs and on the level of serum calcitonin-gene-related peptide (CGRP) and endothelin-1.
METHODS: Fourteen healthy Xishuangbanna Miniature pigs were randomly divided into hormonal group and control group. The brain death model was established by slow intracranial pressure method. The pigs in the hormonal group were performed with intravenous injection of 6 mg/kg methylprednisolone sodium succinate at 30 minutes before establishment, and the pigs in the control group were not treated with hormone. The mean arterial pressure, left ventricular ejection fraction and the CGRP and endothelin-1 level in serum were measured at 0, 1, 3, 6 and 9 hours after brain death.
RESULTS AND CONCLUSION: The mean arterial pressure and left ventricular ejection fraction in the hormonal group were significantly higher than those in the control group (P < 0.05). The level of CGRP was gradually decreased and the level of endothelin-1 was gradually increased in the hormonal group, there was significant difference between hormonal group and control group (P < 0.001). Hormone can protect heart function effectively in the brain dead pig donor, which might be related to the decreasing of inflammatory reaction, the increasing of energy support of heart and maintenance of internal environment stabilization.

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