Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (12): 2197-2202.doi: 10.3969/j.issn.1673-8225.2012.12.025

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Preparation and characterization of porous silk fibroin/chitosan scaffolds

Liu Lei1, Zeng Shu-guang1, Ren Li2, Wei Liu-qiong1, Yang Jing1, Cao Yan-nan1, Shi Yong1   

  1. 1Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou  510280, Guangdong Province, China; 2Department of Materials Science and Engineering, South China University of Technology, Guangzhou  510641, Guangdong Province, China
  • Received:2011-12-16 Revised:2012-01-14 Online:2012-03-18 Published:2012-03-18
  • Contact: Corresponding author: Zeng Shu-guang, Master’s supervisor, Professor, Chief physician, Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, Chinasunrisez@live.cn Corresponding author: Ren Li, Doctor, Professor, Doctoral supervisor, Department of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, Chinapsliren@scut.edu.cn
  • About author:Liu Lei★, Studying for master’s degree, Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China liuleijinhua@163.com
  • Supported by:

    Research Foundation of Guangdong Bureau of Traditional Chinese Medicine, No. 20111145*; Guangdong Science and Technology Program, No.2011B031300022*

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Abstract:

BACKGROUND: Silk fibroin and chitosan are non-toxic and have a good histocompatibility. However, they could not satisfy the biological scaffold material needs if working separately.
OBJECTIVE: To explore and analyze the material structure and relate performance for characterization on the basis of making a variety of compound scaffolds with different proportion of silk fibroin and chitosan, and to select the ideal scaffolds that suitable for the growth of osteoblasts.
METHODS: 2% silk fibroin solution was made by dissolution from CaCl2 solvent systems at a mole ratio of CaCl2:C2H5OH:H2O=  1:2:8, filtration, concentration and purification. The chitosan was dissolved in acetic acid solution prepared 3% chitosan-acetic acid solution. The silk fibroin and chitosan were blend in a freeze-drying approach in different proportion in order to get different scaffold materials. Then the morphology of the silk fibroin/chitosan scaffolds was observed under a scanning electron microscope; the porosity was calculated and the structure of the scaffolds was evaluated and observed by Fourier transform infrared spectroscopy, X-ray diffraction and electron spectroscopy.
RESULTS AND CONCLUSIONS: More stable scaffolds were created with the method of blending and modifying silk fibroin and chitosan. The 40% silk fibroin-60% chitosan group proves the preferred ratio of cell scaffold material due to its better aperture for the growth of osteoblasts.

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