Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (12): 2115-2119.doi: 10.3969/j.issn.1673-8225.2012.12.006

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Injection of polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres into chitin nerve regeneration chamber can promote sciatic nerve regeneration

Huang Ya-zhou, Chen Qing-han, Ren Ming-ming   

  1. Department of Orthopedics, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou  450014, Henan Province, China
  • Received:2011-12-12 Revised:2012-01-14 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Chen Qing-han, Master, Professor, Chief physician, Department of Orthopedics, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan Province, China
  • About author:Huang Ya-zhou, Department of Orthopedics, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan Province, China 1105831335@ qq.com

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Abstract:

BACKGROUND: Erythropoietin (EPO) plays an important role in hematopoiesis as well as in the repair of nervous system injury.
OBJECTIVE: To observe the effect of polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres on sciatic nerve regeneration.
METHODS: Sixty female SD rats were randomly divided into three groups, 20 rats in each group. The bilateral sciatic nerve defect model was prepared (1 cm defect) and the absorbable chitin nerve regeneration chamber was prepared. The chambers of the rats in the experimental group were injected with polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres, chambers of the rats in the control group were injected with polylactic acid-polyglycolic acid microspheres and chambers of the rats in the blank control group were injected with normal saline in the same dose.
RESULTS AND CONCLUSION: The conduction velocity of the regeneration nerve in the experimental group was better than that in the control group and blank control group, and the conduction velocity at the 12th week was better than that at the 6th week. There was significant difference (P < 0.05). S-100 immunohistochemistry and Loyezs neural staining showed that the number of nerve fibers in experimental group was more that in control group and blank control group, and the number of nerve fibers at the 12th week was more than that at the 6th week; the difference was statistically significant (P < 0.05). Polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres has the effect of promoting sciatic nerve regeneration and functional recovery.

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