Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1866-1870.doi: 10.3969/j.issn.1673-8225.2012.10.034

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Diverse expression patterns of microRNA-155 between bone marrow-derived M1 and M2 macrophages  

Liang Yan-bing, Wang Zhong-hua, Tang Hao, Ma Zhong-fu   

  1. Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2012-01-08 Revised:2012-02-04 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Ma Zhong-fu, Professor, Doctoral supervisor, Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China mazhongfu05@sohu.com
  • About author:Liang Yan-bing★, Master, Associate chief physician, Master’s supervisor, Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China xuyw8591@126.com
  • Supported by:

     the Science and Technology Development Program of Guangdong Province, No. 2009B080701070*, 2009B080701004*

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Abstract:

BACKGROUND: Previous studies of microRNA-155 expression and function in macrophage predominantly focus on total mononuclear macrophage.
OBJCTIVE: To evaluate the expression difference of microRNA-155 in M1 and M2 macrophages.
METHODS: Bone marrow-derived macrophages were induced to differentiate towards M1 and M2 macrophages using interferon-γ (100 U/mL) + lipopolysaccharide (5 ng/mL) and interleukin-4 (10 ng/mL), respectively.
RESULTS AND CONCLUSION: The differentiation proportion of M1 and M2 macrophages detected by flow cytometry was 91% and 95%, respectively. Reverse transcription PCR method showed that inducible nitric oxide synthase (iNOS) mRNA was highly expressed in M1 macrophages, but it was hardly expressed in M2 macrophages. Arginase-1 and found in inflammatory zone 1 mRNA expression was highly expressed in M2 macrophages, but the expression was low in M1 macrophages. Tumor necrosis factor α mRNA expression was significantly higher in M1 macrophages than in M2 macrophages, on the contrary, interleukin 10 mRNA expression was significantly higher in M2 macrophages than in M1 macrophages (P < 0.05). Real-time fluorescence quantitative PCR showed that microRNA-155 expression in M1 macrophages was significantly higher compared with that in M2 macrophages (P < 0.05). These findings suggest that MiRNA-155 can serve as a useful maker for differential diagnosis of M1 and M2 macrophages.

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