Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1846-1850.doi: 10.3969/j.issn.1673-8225.2012.10.030

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Effects of ganglioside combined with neural stem cells transplantation on hippocampal neuron following brain injury in rats 

Li Hong-xing1, Zhao Zong-mao2, Jin Yao-dong3, Wang Jing4, Yuan Guo-fu5   

  1. 1Department of Neurosurgery; 3Department of Emergency; 4Department of Pharmacy; 5Department of Brain Surgery, Xushui People's Hospital, Xushui  072550, Hebei Province, China; 2Department of Neurosurgery, Second Hospital of Hebei Medical University, Shijiazhuang  050000, Hebei Province, China
  • Received:2011-09-15 Revised:2011-11-10 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Zhao Zong-mao, Chief physician, Associate professor, Master’s supervisor, Department of Neurosurgery, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
  • About author:Li Hong-xing, Attending physician, Department of Neurosurgery, Xushui People's Hospital, Xushui 072550, Hebei Province, China
  • Supported by:

    Key Subjects of Medical Research in Hebei Province, No. 20110598*; Outstanding Youth Foundation of Hebei Province, No.L2009001547*; Hebei Science and Technology Support Program, No.09276101D-35*

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Abstract:

BACKGROUND: The research about the application of neural stem cells (NSCs) transplantation on the treatment of neurological dysfunction after brain injury is a hot spot.
OBJECTIVE: To investigate the effect of ganglioside combined with NSCs transplantation on hippocanipal neuron following traumatic brain injury (TBI) in rats．
METHODS: Sixty-six healthy Wistar rats were selected to establish the hippocanipal neuron model cause by TBI using improved Feeney method. After injured for 24 hours, the surviving 60 rats were randomly divided into 3 groups, the TBI group was injected with 1 mL DMEM/F12 medium; the NSCs group was injected with 1×1010/L NSCs suspension; the NSCs+ganglioside group was injected with 1×1010/L NSCs suspension and then intraperitoneal injection of 30 mg/kg ganglioside aqueous solution, once per day and lasted for 3 days. 
RESULTS AND CONCLUSION: Fluorescence microscope observation showed that the NSCs labeled by PKH26 were spherical and presented with red and uniform-distributed fluorescence. Flow cytometric detection revealed that the positive percentage of PKH26 labeled NSCs was over 95%. The average time of escape latency was gradually decreased in each group. However, from the 3rd to 5th day, time in NSCs+ganglioside group was shorter than that in NSCs transplantation group (P < 0.05) and compared with TBI group, time in NSCs+ganglioside group was decreased significantly (P < 0.01). In addition, the frequency of platform passing and the percentage of swimming distance in target quadrant and the total distance were highest in NSCs+ganglioside group (P < 0.05). The number of PKH26-positive cells and neurons in the section stained by hematoxylin-eosin as well as the mRNA expression of brain-derived neurotrophic factor in NSCs+ganglioside group was more than that in NSCs group, and those factors in NSCs group were more than those in TBI group (P < 0.05). NSCs transplantation plays a role in protecting against hippocampal neuronal death following TBI, with the affiliation of ganglioside claim synergism effects.
 

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