Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1822-1826.doi: 10.3969/j.issn.1673-8225.2012.10.025

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Effect of 5-fluorouracil on the expression of Oct4 and Bmi-1 in human breast cancer cell line MCF7  

Yan Li1, Shi Li-Gang1, Xu Zheng-Shun1, Li Hui-Xiang2   

  1. 1College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan Province, China; 2Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
  • Received:2011-09-03 Revised:2012-01-08 Online:2012-03-04 Published:2012-03-04
  • About author:Yan Li★, Master, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan Province, China yanli0546@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30971149*

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Abstract:

BACKGROUND: Several studies suggest that occurrence and development of breast cancer is a progressional process in which many factors participate, and breast cancer stem cells are thought to be the root cause that may initiate the growth of breast cancer recurrence and far metastasis after neoadjuvant chemotherapy.
OBJECTIVE: To investigate the influence of 5-fluorouracil (5-Fu) on the expression of Oct4 and Bmi-1 in human breast cancer cell line MCF7.
METHODS: MCF7 was treated with 0.1 mg/L 5-Fu, the expression of Oct4 and Bmi-1 in untreated cells and six generations of treated cells was detected with reverse transcription-polymerase chain reaction, and the expression of Oct4 in untreated cells and five generations of treated cells was detected by immunocytochemistry.
RESULTS AND CONCLUSION: After 5-Fu treatment, Oct4 and Bmi-1 mRNA expression in the third and fifth generations of treated cells was significantly increased, and Oct4 protein during first to fifth generations of cells had a certain degree of regularity. These findings suggest that there may be cancer stem cell-like cells in MCF7 and 5-Fu might increase the number of cancer stem cell-like cells in MCF7.

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