Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1817-1821.doi: 10.3969/j.issn.1673-8225.2012.10.024

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Human bone morphogenetic protein-2 recombinant adenovirus expression vector transfection of rabbit bone marrow mesenchymal stem cells   

He Chun-lei, Ji Guang-lin, Liu Wu-yang, Wu Dong-bao, He Cheng, Wang Mao-yuan, Ye Yong-jun, Mo Jian-wen, Gao Hui   

  1. Department of Orthopedics, First Affiliated Hospital of Gannan Medical College, Ganzhou  341000, Jiangxi Province, China
  • Received:2011-08-08 Revised:2011-09-22 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Gao Hui, Professor, Mater’ s supervisor, Department of Orthopedics, First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China gaohui3721@163.com
  • About author:He Chun-lei★, Master, Physician, Department of Orthopedics, First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China chunleihe1983@126.com
  • Supported by:

    Science and Technology Project of Jiangxi Education Bureau, No. GJJ09327*

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Abstract:

BACKGROUND: The adenovirus vector structured by GatewayTM has made up the deficiencies of exogenous cytokine application. 
OBJECTIVE: To observe the target gene expression and osteoblastic differentiation of human bone morphogenetic protein-2 (hBMP-2) recombinant adenovirus expression vector (Ad-BMP-2/GFP) after transfected with bone marrow-derived mesenchymal stem cells (rBMSCs).
METHODS: To isolate and culture, purify rBMSCs through the density gradient centrifugation combined with adherent cultivation; the Ad-BMP-2/GFP adenovirus vector constructed by GatewayTM technology was transfected with rBMSCs.
RESULTS AND CONCLUSION: Osteocalcin was the osteoblast-specific product which could be expressed on the transfected rBMSCs detected by RT-PCR method; hBMP-2 gene was positive at mRNA and protein levels. Rabbit bone marrow stromal cells were successfully separated and purified. Ad-BMP-2 can efficiently transfect rBMSCs and the BMP-2 can be expressed in BMSCs efficiently.

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