Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1808-1812.doi: 10.3969/j.issn.1673-8225.2012.10.022

Previous Articles     Next Articles

Effects of Dlx2 overexpression on cell cycle regulation and apoptosis of preosteoblast cells

Sun Hao, Wang Xu-dong, Dai Jie-wen, Lu Jing-ting, Shen Guo-fang   

  1. Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai  200011, China
  • Received:2011-06-04 Revised:2011-09-09 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Wang Xu-dong, Associate professor, Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China xudongwang70@hotmail.com
  • About author:Sun Hao★, Master, Physician, Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China sunhaohappy@yahoo.com.cn
  • Supported by:

    Shanghai Leading Academic Discipline Project, No. S30206*; Research Fund of Science and Technology Commission of Shanghai City, No. 10JC1408700*; the Natural Science Foundation of Shanghai, No. 10ZR1418000*; Research Fund of Shanghai Municipal Health Bureau, No. 2009077*; Combined Engineering and Medicine Project of Shanghai Jiao Tong University, No. YG2010MS55*

PDF

881

Abstract:

BACKGROUND: Dlx2 plays an important role in the regulation of cranial neural crest cells migration and craniofacial skeleton development. However, the effects of Dlx2 on apoptosis and cell cycle in osteoblast differentiation are not reported.
OBJECTIVE: To investigate the effects of Dlx2 overexpression on cell cycle regulation and apoptosis in MC3T3-E1 preosteoblast cells differentiation.
METHODS: Anti-retroviral pMSCV-puro-Dlx2 was transfected into MC3T3-E1 cells which were cultured in mineralization induced fluid. MC3T3-E1-Dlx2 cell lines stably overexpressing Dlx2 were constructed. Cell lines of Dlx2 over-expression were determined by RT-PCR and western blot. Apoptosis was detected by flow cytometry after Annexin V/PI and PI/RNase staining and cell cycle changes were detected by flow cytometry after PI/RNase staining.
RESULTS AND CONCLUSION: MC3T3-E1-Dlx2 cell lines was successfully constructed. Cell cycle was blocked in G1/G0 phase (P < 0.05) and apoptosis was up-regulated (P < 0.05) in Dlx2 overexpression cell model. Dlx2 overexpression reduces cell proliferation and promotes cell differentiation to exercise functions.

CLC Number: