Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1780-1785.doi: 10.3969/j.issn.1673-8225.2012.10.016

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Biological characteristics and optimal strategy of human amniotic fluid-derived mesenchymal stem cells cultured in vitro   

Wang Pei-pei1, Zheng Yu-bao1, Peng Liang1, Yu Jun2, Gao Zhi-liang1   

  1. 1Department of Infectious Diseases, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou  510630, Guangdong Province, China; 2Department of Infectious Diseases, Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-08-01 Revised:2011-09-01 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Gao Zhi-liang, M.D., Chief physician, Doctoral supervisor, Department of Infectious Diseases, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China gaozl@21cn.com
  • About author:Wang Pei-pei★, Studying for master’s degree, Department of Infectious Diseases, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China smilewangpeipei@163.com
  • Supported by:

    2011-09-01 the National Natural Science Foundation of China, No. 30971356*; the Natural Science Foundation of Guangdong Province, No. 104510089010 04818*; National Eleventh Five-Year Program of China, No. 2008ZX10002-005*

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Abstract:

BACKGROUND: At present, seed sources of stem cells engineering are diverse, and mesenchymal stem cells can be isolated from exfoliated cells in fetal amniotic fluid.
OBJECTIVE: To investigate the effects of the media with different elements on human amniotic fluid-derived mesenchymal stem cells (h-AFMSCs) cultured in vitro, and to analyze the biological characterization of h-AFMSCs at optimum medium cultured in vitro in order to optimize the culture strategy of h-AFMSCs.
METHODS: Amniotic fluid was extracted from pregnant women during 16 to 24 weeks of gestation, and then was used for primary culture and subculture. h-AFMSCs were cultured in media containing six different elements.
RESULTS AND CONCLUSION: h-AFMSCs were isolated from amniotic fluid in mid-trimester. The proliferative ability of h-AFMSCs was improved to a maximal extent in the mixed medium of low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and MESEN PRO medium at a mixed ratio of 1:1. h-AFMSCs highly expressed CD29, CD73, CD90, CD105, CD166 and lowly expressed CD14, CD34, CD45, HLA-DR. Stem cells gene OCT-4 and Naong highly expressed in h-AFMSCs cultured in vitro. h-AFMSCs showed a higher osteogenic and adipogenic differentiation potential, and they could inhibit the proliferation of lymphocytes in vitro. It is indicated that mid-trimester amniotic fluid is a good cell source of h-AFMSCs with lower immunogenicity.

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