Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1769-1772.doi: 10.3969/j.issn.1673-8225.2012.10.014

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Human umbilical cord stromal stem cells-conditioned medium down-regulated the expression of transforming growth factor beta in rat hepatic stellate cells

Li Ming-liang, Yao Zhi-cheng, Zhong Yue-si, Lin Nan, Xu Rui-yun, Deng Mei-hai   

  1. Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou  510630, Guangdong Province, China
  • Received:2011-10-31 Revised:2011-12-16 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Deng Mei-hai, M.D., Chief physician, Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China drdmh@medmail.com.cn
  • About author:Li Ming-liang★, Studying for master’s degree, Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China lmlgz@126.com
  • Supported by:

     the Natural Science Foundation of Guandong Province, No.8151008901000112*; Doctoral Launched Project, Natural Science Foundation of Guandong Province, No.10451008901004752*; Youth Program of the National Natural Science Foundation of China, No.81000177*, 81000674*

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Abstract:

BACKGROUND: Human umbilical cord stromal stem cells are expected to become a new seed cell in the treatment of live cirrhosis. However, few studies of in vitro experiment have been reported.
OBJECTIVE: To explore the effect of human umbilical cord stromal stem cells-conditioned medium (USSC-CM) on the biological activity of fibrosis in rat hepatic stellate cells (HSCs), and the acting target.
METHODS: HSCs were seeded in the six orifice plate in proportion (1.0×105 /mL), then cultured without serum for 24 hours;
2 mL USSC-CM were added to treat HSCs as experimental group; while HSCs were treated with equivalent fresh medium as control group. The expression of transforming growth factor β1 (TGF-β1), collagen-α1 and TIMP-2 mRNA in HSCs were detected by RT-PCR. The expression of TGF-β1 protein was determined by Western blot.
RESULTS AND CONCLUTION: The expression of TGF-β1, collagen-α1 and TIMP-2 mRNA in HSCs were down-regulated in experimental group compared to control group, and the tendency enhanced with time (USSC-CM treated 72 h > USSC-CM treated 48 h > control group). And the expression of TGFβ1 protein in HSCs were down-regulated in experimental group compared to control group and showed time dependent manner (control group > USSC-CM treated 48 h group > USSC-CM treated 72 h group, P < 0.05). Human umbilical cord stromal stem cells can inhibit biological activity of HSCs. And it may carry its point by focusing on the acting target related to TGF-β together with downstream production.

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