Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1765-1768.doi: 10.3969/j.issn.1673-8225.2012.10.013

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Human umbilical cord mesenchymal stem cells influence the proliferation and apoptosis of endometrial cells 

Xu Li-nan1, Xu Bing-nan2, Chen Shu-qin1, He Ke1, Cai Jian1   

  1. 1Department of Gynaecology and Obstetrics, First Affiliated Hospital of Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China;    2Department of Gynaecology and Obstetrics, First People’s Hospital of Foshan, Foshan   528000, Guangdong Province, China
  • Received:2012-01-16 Revised:2012-01-29 Online:2012-03-04 Published:2012-03-04
  • About author:Xu Li-nan☆, M.D., Attending physician, Department of Gynaecology and Obstetrics, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China drxulinan@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No.81070472*; Science and Technology Development Program of Guangdong Province, No.2010B031600313*

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Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) can regulate cellular biological activities by paracrine mechanism.
OBJECTIVE: To investigate the effects of hUCMSCs on the proliferation and apoptosis of endometrial cells.
METHODS: Endometrial cells and hUCMSCs were cultured in vitro. hUCMSCs and endometrial cells were co-cultured on the Transwell co-culture plate at a proportion of 1×105/1×105 to establish upper and lower double-cell non-contact co-culture system, serving as the experimental group. Endometrial cells (1×105 cells/well) were separately cultured as control group.
RESULTS AND CONCLUSION: MTT assay results showed that cell proliferation in the experimental group was significantly inhibited in a time-dependent manner compared with the control group (P < 0.05). Flow cytometry results showed that apoptotic cells were significantly more in the experimental group than in the control group (P < 0.05), and endometrial cells entering into S stage from G1 stage were reduced. RT-PCR results showed that PTEN mRNA expression was significantly higher in the experimental group than in the control group. These findings suggest that hUCMSCs inhibit the in vitro proliferation of human endometrial cells and promote their apoptosis by paracrine mechanism, which occurs possibly by upregulation of PTEN mRNA expression.

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