Abstract:

BACKGROUND: In vivo experiment has shown that the microenvironment of bone marrow mesenchymal stem cells (BMSCs) plays an important role on induced differentiation.
OBJECTIVE: To in vitro induce the BMSCs to differentiate into cardiac tissue-like structure.
METHODS: Changes of morphological characteristics of the third generation of BMSCs that cultured with myocardial tissue lysates for 4 weeks were observed under inverted microscope. The tissue was collected and preformed with hematoxylin-eosin staining, and the ultrstructure of the induced cells was observed by electron microscope. Immunohistochemical staining was preformed using vimentin and Ⅷ factor-related antigen.
RESULTS AND CONCLUSION: Myocardial tissue lysates were cultured for 4 weeks. At the 12th day, some “bamboo-like” cells were fused to form multinucleated myotube-like structure. From the 14th day, rhythmic contraction could be observed with the beating frequency about 90-110/min; sarcomere-like structure of filaments could be seen under transimission electron microscope and some muscle cells had the function of automatic pulsating. Hematoxylin-eosin staining showed a large number of bundle-like structures. Myocardial tissue lysates can be used as simulated cardiac tissue microenvironment inducer to promote the BMSCs to differentiate into myocardial cells with typical morphological features and the corresponding functions, and induce part of the BMSCs to differentiate into connective tissue cells, endothelial like cells, and form cardiac tissue-like structure together with cardiac cells. BMSCs have the multiple differentiation potential, forming a variety of cells with similar functions and forming the tissue-like structure trend in a certain microenvironment.