Abstract:

BACKGROUND: Is it positively or negatively correlated with osteoblastic cytokine secretion by adding bioactive glass ion to osteoblast culture fluid?
OBJECTIVE: To observe the gene expression of type Ⅰ collagen and bone gla-protein (BGP) of osteoblasts after addition with bioactive glass in order to further investigate the possible mechanisms of bioactive glass promoting osteoblast proliferation.
METHODS: The full Dulbecco’s modified eagle medium (DMEM) was prepared as the control group culture fluid, and the full DMEM culture with ionic products of bioactive glass dissolution was prepared to be the experimental group culture fluid. The second filial generation osteoblastes isolated from mouse cranial bone were cultured by two kinds of culture fluid respectively for  4 days and then, an experimental observation was done.
RESULTS AND CONCLUSION: The ratio of experimental group to control group in the gene expression quantity of the two mice osteoblast cell factors was as follows: type Ⅰ collagen=3.376 and BGP=2.687. There was significant difference in the relative amount to the inner reference gene between experimental group and control group (P < 0.05). Bioactive glass promotes the gene expression of type Ⅰ collagen and BGP in osteoblasts, which is beneficial to matrix formation and mineralization around the osteoblasts during bone repairing process, and thus promotes the bone healing.