Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1331-1335.doi: 10.3969/j.issn.1673-8225.2012.08.001

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Three-dimensional porous poly(L-lactic acid)/chitosan nanofiber scaffolds combined with bone marrow mesenchymal stem cells in repairing of bone defects

Wang Huan1, 2, Lin Hong-sheng1, 2, Zha Zhen-gang1, 2, Tan Wen-cheng3△, Zheng Li-heng4△, Yao Ping5, Zhang Jia-qing6,
Miao Gui-qiang7   

  1. 1Institute of Bone Disease, Jinan University, Guangzhou  510632, Guangdong Province, China; 2Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou  510630, Guangdong Province, China; 3Renhe Medical Center of Macao, Macao Special Administrative Region  999078, China; 4Hospital Conde S. of Macao, Macao Special Administrative Region  999078, China; 5Department of Physiology,      6Department of Biochemistry, Jinan  University Medical School, Guangzhou  510632, Guangdong Province, China;    7People's Hospital of Nanhai District of Foshan, Foshan  528200, Guangdong Province, China
  • Received:2011-11-20 Revised:2011-12-14 Online:2012-02-19 Published:2012-02-19
  • Contact: Lin Hong-sheng, Professor, Doctoral supervisor, Institute of Bone Disease, Jinan University, Guangzhou 510632, Guangdong Province, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China tlinhsh@jnu.cn
  • About author:Wang Huan★, Studying for master’s degree, Institute of Bone Disease, Jinan University, Guangzhou 510632, Guangdong Province, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China illiyu@qq.com
  • Supported by:

    National 863 High Technology Research and Development Program of China, No.2007AA09Z440*; Macao Science and Technology Development Foundation, No.031/2009/A*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have the ability to differentiate to a variety of mesenchymal cell lineages, and the properties of scaffold material have a great impact on bone defects.
OBJECTIVE: To observe the possibility of repairing bone defects by three-dimensional porous poly(L-lactic acid)/chitosan nanofiber (PLLA/CSNF) scaffolds combined with BMSCs.
METHODS: The rabbit bone defect models were implanted with posterior superior iliac spine autogenous cancellous bone (cancellous bone group), PLLA/CSNF porous scaffold and the PLLA/CSNF porous scaffold combined with BMSCs (BMSCs+PLLA/CSNF group). The model group was without implantation.   
RESULTS AND CONCLUSION: Bone tissue was formed in the defected position in the BMSCs+PLLA/CSNF group after implanted for 12 weeks, the scaffolds were degraded and the repair of the defect was completed, the effect of repairing defects was similar to that in the cancellous bone group; in the cancellous bone group, the defects were healing well and the newly formed bone tissue were arranged in order; some new bone tissue were formed and the scaffolds were degraded partly in PLLA/CSNF group while the defect was mainly filled with fibrous connective tissue in model group and there was no new bone tissue formation. The three-dimensional porous PLLA/CSNF scaffolds co-cultured with BMSCs implanted into the defects of the femoral condyle could increase the speed of repairing and possesses a superior osteoinductivity in vivo.

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