Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1323-1326.doi: 10.3969/j.issn.1673-8225.2012.07.043

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Correlation between stromal cell-derived factor-1 and matrix metalloproteinase 3, 9 and 13 in the synovial fluid of knee osteoarthritis patients after injection of sodium hyaluronate  

Li Yan-lin, Wang Guo-liang, Cao Bin, Ma Ke, Xu Peng, Yang Guang, Gao Gang   

  1. Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-08-02 Revised:2011-11-16 Online:2012-02-12 Published:2012-02-12
  • About author:Li Yan-lin☆, Doctor, Professor, Doctoral supervisor, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China yanlinli1969@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 30860286*

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Abstract:

BACKGROUND: Sodium hyaluronate localized in the synovial fluid and cartilage of knee joint plays an important role in the occurrence and development of osteoarthritis. Recent studies have shown that stromal cell-derived factor-1, matrix metalloproteinases 3, 9 and 13 affect the cartilage degeneration in osteoarthritis.
OBJECTIVE: To explore the effects of intra-articular injection of sodium hyaluronate on the expression levels of stromal cell-derived factor 1 and matrix metalloproteinases 3, 9 and 13 in the knee joint fluid of patients with osteoarthritisn and the correlation between them.
METHODS: A total of 20 cases of knee osteoarthritis patients with synovial fluid were involved in this study. Joint fluid was extracted before the injection of sodium hyaluronate, in the 1st, 2nd, 3rd and 4th weeks after injection. The levels of stromal cell-derived factor 1, matrix metalloproteinases 3, 9 and 13 in the synovial fluid were measured by double antibody sandwich ELISA assay. The correlation between them was analyzed.
RESULTS AND CONCLUSION: After intra-articular injection of sodium hyaluronate, the level of stromal cell-derived factor 1, matrix metalloproteinases 3, 9 and 13 in the synovial fluid decreased. In the groups of stromal cell-derived factor 1, matrix metalloproteinases 3 and 9, there was no significant difference before injection and in 1 week after injection, 1 week after injection and 2 weeks after injection (P > 0.05). There was significant difference in other groups compared with each other (P < 0.05). The correlation between stromal cell-derived factor 1 and matrix metalloproteinases 3, 9 and 13 was positive.

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