Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1245-1250.doi: 10.3969/j.issn.1673-8225.2012.07.025

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Expression of nuclear factor kappa B1 and tissue factor in femoral venous endothelial tissue of the rat deep vein thrombosis model******☆

Li Xing-guo1 , Zheng Hong-yu1, Li Wen2, Li Hong-kun1, Zhao Xue-ling1, Wu Xue-mei1, Wang Bing1   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China; 2Department of Cardiology, Yunnan Provincial People’s Hospital, Kunming  650032, Yunnan Province, China
  • Received:2011-11-12 Revised:2011-12-12 Online:2012-02-12 Published:2012-02-12
  • Contact: Wang Bing, Doctor, Associate chief physician, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China wangbingdoctor@126.com Wu Xue-mei, Nurse-in-charge, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China ynwuxuemei@126.com
  • About author:Li Xing-guo,☆ Studying for doctorate, Lecturer, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China ynwuxuemei@126.com Zheng Hong-yu, Studying for doctorate, Physician, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30960389*, 81060151*, 81160236*; Joint Funds of Yunnan Sci-Tech Department and Kunming Medical College, No. 2009cd159*; Yunnan New Key Product Development Projects, No. 2010BC010*; Doctor Innovation Foundation of Kunming Medical College, No. 2011D07*

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Abstract:

BACKGROUND: At present, the basic underlying molecular mechanism regulating the interactions among venous endothelial cells, platelets, leukocytes, and promoting local deep vein thrombosis microenvironment formation, still remains unclear, and there is no ideal method for early diagnosis of deep vein thrombosis.
OBJECTIVE: To study the underlying role of nuclear factor kappa B1 and tissue factor in rats with deep vein thrombosis.
METHODS: A total of 67 Sprague-Dawley rats were randomly divided into control group (n=10) and model group (n=57). Deep vein thrombosis model was established by a clamping and sewing method in femoral vein combined with cast fixation. The incidence and serious degree of thrombus were observed by dissecting rat femoral vein in different time points (2.5 and 25 hours after modeling). The model group was further divided into pre-thrombogenesis group (2.5 hours after modeling), thrombogenesis group (25 hours after modeling) and non-thrombogenesis group (25 hours after modeling). Then total RNA was extracted from the localized femoral venous endothelial tissue. The candidate genes, associated inflammation and thrombosis, were screened by a special gene chip. Then the gene expression of nuclear factor kappa B1 and tissue factor was further identified by real-time polymerase chain reaction.
RESULTS AND CONCLUSION: Pre-thrombogenesis group had no thrombogenesis, while thrombogenesis group have 23 cases with thrombosis and non-thrombogenesis group have 22 cases without thrombosis. The results of gene chip hybridization analysis and real-time PCR found that the mRNA expression of nuclear factor kappa B1 and tissue factor in rat femoral vein endothelial tissue were significantly up-regulated at 2.5 hours after modeling (pre-thrombogenesis group was higher than control group) (P < 0.05), and continued up-regulating at 25 hours after modeling (thrombogenesis group was higher than the pre-thrombogenesis group, non-thrombogenesis group and control group) (P < 0.05). The results from present study indicate that up-regulating expressions of nuclear factor kappa B1 and tissue factor in local femoral venous endothelial tissue of rat deep vein thrombosis models may play a key role in initiating venous thrombosis.

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