Abstract:

BACKGROUND: Previously, sub-health and chronic fatigue model is constructed with complex physical factors, but sub-health fatigue rat model is rarely report.
OBJECTIVE: To observe the sub-health fatigue rat models constructed by sleep deprivation combined with exhaustive weight-loading swimming exercise.
METHODS: Thirty-two male rats were randomly divided into four groups by weight. Control group, 1-day model, 4-day model and 5-day model group. Rats in the model group were used to establish the model of sub-health fatigue by being deprived of sleep for 20 hours, and then the rats were required to conduct exhaustive swimming exercise with loading 5% of rat body weight. The exhaustive swimming time was recorded. Each group was preformed with fasting for one day in the end of the experiment, and toke the blood biochemistry.
RESULTS AND CONCLUSION: Compared the last exhaustive swimming time with the first, the exhaustive swimming time of 4-day model and 5-day model group was shorter significantly (P < 0.05). The levels of blood Alpha-hydroxybutyrate dehydrogenase and blood urea nitrogen in 5-day group was increased compared with the rest groups (P < 0.05). The levels of blood cholesterol aspartate aminotransferase, alanine aminotransferase, creatinine and triglyceride were decreased (P < 0.05). Exhaustive swimming exercise with loading 5% of rat body weight and sleep deprivation for 20 hours might establish a rat model of sub-health fatigue.