Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1220-1224.doi: 10.3969/j.issn.1673-8225.2012.07.019

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Verification of differential expression of angiogenesis related genes in normal tissues and myocardial infarction tissues of rat models of myocardial infarction using real-time PCR method

Yu Li-wei, Jiang Meng, Zhu Dan, Yu Jie-jing, Ma Jun   

  1. Department of Cardiology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai  200001, China
  • Received:2011-10-08 Revised:2011-12-05 Online:2012-02-12 Published:2012-02-12
  • Contact: Jiang Meng, Doctor, Department of Cardiology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China jiangmeng0919@163.com
  • About author:Yu Li-wei,★ Studying for master’s degree, Department of Cardiology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China yuliwei19@hotmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30800453*; the Rising Star Program of Science and Technology for Youth in Shanghai, No. 10QA1404500*; the Pilot Project of Innovation for Students of Shanghai Jiao Tong University School of Medicine, No. IAP3117*; the Cross Project Biomedical Engineering of Shanghai Jiao Tong University, No. YG2010MS29* 

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Abstract:

BACKGROUND: Researches have shown that the process of angiogenesis after acute myocardial infarction is mediated by a number of genes.
OBJECTIVE: To investigate the differential expression of angiogenesis related genes after acute myocardial infarction.
METHODS: Rat model of acute myocardial infarction was constructed; five specific differential expression genes in gene chip were selected based on the results of gene chips: secreted phosphoprotein 1, chemokine receptor 2, angiopoietin-like 4, CXC chemokine ligand 5 and interleukin-1β. Real-time PCR was conducted to detect the expression levels of these genes in normal and in infarction myocardial tissues of rat acute myocardial infarction model.
RESULTS AND CONCLUSION: The gene expression of the secreted phosphoprotein 1, chemokine receptor 2 and angiopoietin-like 4 increased significantly in the early stage of acute myocardial infarction (P < 0.05); and it was time-dependent. While the expression levels of CXC chemokine ligand 5 and interleukin-1β showed low fluctuation without significant changes. Among them, the secreted phosphoprotein 1 and chemokine receptor 2 are related to the inflammation, cell adhesion, migration and chemotaxis after acute myocardial infarction; while the angiopoietin-like 4 is related to angiogenesis and cell differentiation.

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